Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

TY - JOUR

T1 - Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

AU - Ali, Chaudhry Haider

AU - Mbadinga, Serge Maurice

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2015 Taiwan Institute of Chemical Engineers.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Lipase from the newly isolated Pseudomonas aeruginosa strain was produced, and its activity was enhanced through response surface methodology optimization. In the initial screening process, the bacterial strain was isolated from soil samples and then confirmed with lipase production on agar plates supplemented with Rhodamine B. The strain was identified by 16S rRNA gene sequence analysis. Plackett-Burman design was used to initially screen nine factors of culture medium. The design showed that four factors including peanut oil, sucrose, ammonium sulfate and volume of the medium exhibited significant affect on lipase production by P. aeruginosa strain. Afterward the optimal level of each significant factor was derived by Box-Behnken design and a 13.7 fold increase in lipase activity (21U/mL-288U/mL) was achieved under the optimum conditions. Thirty six percent of the soybean oil was converted into free fatty acids after hydrolyzing the oil for 12h in the presence of optimized lipase.

AB - Lipase from the newly isolated Pseudomonas aeruginosa strain was produced, and its activity was enhanced through response surface methodology optimization. In the initial screening process, the bacterial strain was isolated from soil samples and then confirmed with lipase production on agar plates supplemented with Rhodamine B. The strain was identified by 16S rRNA gene sequence analysis. Plackett-Burman design was used to initially screen nine factors of culture medium. The design showed that four factors including peanut oil, sucrose, ammonium sulfate and volume of the medium exhibited significant affect on lipase production by P. aeruginosa strain. Afterward the optimal level of each significant factor was derived by Box-Behnken design and a 13.7 fold increase in lipase activity (21U/mL-288U/mL) was achieved under the optimum conditions. Thirty six percent of the soybean oil was converted into free fatty acids after hydrolyzing the oil for 12h in the presence of optimized lipase.

KW - Hydrolysis

KW - Lipolytic activity

KW - Plackett-Burman design

KW - Pseudomonas aeruginosa

KW - Response surface methodology

KW - Vegetable oils

UR - http://www.scopus.com/inward/record.url?scp=84930575548&partnerID=8YFLogxK

U2 - 10.1016/j.jtice.2015.02.001

DO - 10.1016/j.jtice.2015.02.001

M3 - 文章

AN - SCOPUS:84930575548

SN - 1876-1070

VL - 52

SP - 7

EP - 13

JO - Journal of the Taiwan Institute of Chemical Engineers

JF - Journal of the Taiwan Institute of Chemical Engineers

ER -