Salmonella induces the formation of filamentous structures containing lysosomal membrane glycoproteins in epithelial cells

TY - JOUR

T1 - Salmonella induces the formation of filamentous structures containing lysosomal membrane glycoproteins in epithelial cells

AU - Garcia-del Portillo, F.

AU - Zwick, M. B.

AU - Ka Yin Leung, Yin Leung

AU - Finlay, B. B.

PY - 1993

Y1 - 1993

N2 - Salmonella species invade and replicate within epithelial cells in membrane-bound vacuoles. In this report we show that upon infection of HeLa epithelial cells, Salmonella typhimurium resides in vacuoles that contain lysosomal membrane glycoproteins (Igps). Four to six hours after invasion, intracellular bacteria induce the formation of stable filamentous structures containing Igps that are connected to the bacteria-containing vacuoles. Formation of these Igp-rich structures requires viable intracellular bacteria and is blocked by inhibitors of vacuolar acidification. These structures are not present in uninfected cells or in cells infected with another invasive bacteria, Yersinia enterocolitica. Tracers added to the extracellular medium are not delivered to the Salmonella-induced filaments, suggesting that these structures are different from previously described tubular lysosomes. Initiation of intracellular bacterial replication correlates with formation of these Igp-containing filaments. Certain avirulent Salmonella mutants that are defective for intracellular replication fail to induce formation of these structures. These observations suggest that Salmonella-induced filaments containing Igps are linked to intracellular bacterial replication.

AB - Salmonella species invade and replicate within epithelial cells in membrane-bound vacuoles. In this report we show that upon infection of HeLa epithelial cells, Salmonella typhimurium resides in vacuoles that contain lysosomal membrane glycoproteins (Igps). Four to six hours after invasion, intracellular bacteria induce the formation of stable filamentous structures containing Igps that are connected to the bacteria-containing vacuoles. Formation of these Igp-rich structures requires viable intracellular bacteria and is blocked by inhibitors of vacuolar acidification. These structures are not present in uninfected cells or in cells infected with another invasive bacteria, Yersinia enterocolitica. Tracers added to the extracellular medium are not delivered to the Salmonella-induced filaments, suggesting that these structures are different from previously described tubular lysosomes. Initiation of intracellular bacterial replication correlates with formation of these Igp-containing filaments. Certain avirulent Salmonella mutants that are defective for intracellular replication fail to induce formation of these structures. These observations suggest that Salmonella-induced filaments containing Igps are linked to intracellular bacterial replication.

UR - http://www.scopus.com/inward/record.url?scp=0027375512&partnerID=8YFLogxK

U2 - 10.1073/pnas.90.22.10544

DO - 10.1073/pnas.90.22.10544

M3 - 文章

C2 - 8248143

AN - SCOPUS:0027375512

SN - 0027-8424

VL - 90

SP - 10544

EP - 10548

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 22

ER -