Responses of aerobic and anaerobic ammonia/ammonium-oxidizing microorganisms to anthropogenic pollution in coastal marine environments

TY - CHAP

T1 - Responses of aerobic and anaerobic ammonia/ammonium-oxidizing microorganisms to anthropogenic pollution in coastal marine environments

AU - Cao, Huiluo

AU - Li, Meng

AU - Dang, Hongyue

AU - Gu, Ji Dong

N1 - Funding Information: This work was supported in part by grants from Agriculture, Fisheries and Conservation Department of the Hong Kong SAR Government (J.-D. G), a PhD studentship from the University of Hong Kong to Huiluo Cao and Meng Li; China National Natural Science Foundation grants 91028011, 41076091, and 40576069, China Ocean Mineral Resources R&D Association grants DYXM-115-02-2-20 and DYXM-115-02-2-6, Hi-Tech Research and Development Program of China grant 2007AA091903, Fundamental Research Funds for the Central Universities of China grant 09CX05005A, the National Qingdao Economic and Technological Development Area Science and Technology Development Project grant (2009-2-34), and Foundation of the State Key Laboratory of Heavy Oil Processing, China University of Petroleum grant SKL2010-02 to Hongyue Dang. We would like to thank Martin G. Klotz for his vision, patience, and guidance throughout this project.

PY - 2011

Y1 - 2011

N2 - Up to date, numerous studies have shown that the community structure of aerobic ammonia oxidizers including ammonia-oxidizing Betaproteobacteria (Beta-AOB) and ammonia-oxidizing archaea (AOA) and, more recently, the anaerobic ammonium-oxidizing (anammox) bacteria is responsive to environmental conditions including salinity, pH, selected metal ions, concentrations of inorganic nitrogen, total phosphorus, the ratio of organic carbon and nitrogen, and sedimentological factors such as the sediment median grain size. Identification of these responses to known anthropogenic pollution is of particular interest to better understand the growth dynamics and activities of nitrogen transforming microorganisms in marine environments. This chapter discusses currently available methods including molecular ecological analysis using clone library constructions with specific molecular genetic markers for delineating community changes of Beta-AOB, AOA, and anammox bacteria. Using data on ammonia-oxidizing microbial community structures from Jiaozhou Bay in North China and three marine environments with anthropogenic pollution gradients in South China from coastal Mai Po Nature Reserve of Hong Kong to the South China Sea as examples, statistical analyses packages (DOTUR, UniFrac, and Canoco) are presented as useful tools to illustrate the relationship between changes in nitrogen metabolizing microbial communities and established environmental variables.

AB - Up to date, numerous studies have shown that the community structure of aerobic ammonia oxidizers including ammonia-oxidizing Betaproteobacteria (Beta-AOB) and ammonia-oxidizing archaea (AOA) and, more recently, the anaerobic ammonium-oxidizing (anammox) bacteria is responsive to environmental conditions including salinity, pH, selected metal ions, concentrations of inorganic nitrogen, total phosphorus, the ratio of organic carbon and nitrogen, and sedimentological factors such as the sediment median grain size. Identification of these responses to known anthropogenic pollution is of particular interest to better understand the growth dynamics and activities of nitrogen transforming microorganisms in marine environments. This chapter discusses currently available methods including molecular ecological analysis using clone library constructions with specific molecular genetic markers for delineating community changes of Beta-AOB, AOA, and anammox bacteria. Using data on ammonia-oxidizing microbial community structures from Jiaozhou Bay in North China and three marine environments with anthropogenic pollution gradients in South China from coastal Mai Po Nature Reserve of Hong Kong to the South China Sea as examples, statistical analyses packages (DOTUR, UniFrac, and Canoco) are presented as useful tools to illustrate the relationship between changes in nitrogen metabolizing microbial communities and established environmental variables.

UR - http://www.scopus.com/inward/record.url?scp=79955487255&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-386489-5.00002-6

DO - 10.1016/B978-0-12-386489-5.00002-6

M3 - 章节

C2 - 21514459

AN - SCOPUS:79955487255

T3 - Methods in Enzymology

SP - 35

EP - 62

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -