Observer-based non-linear control of a fed-batch autoinductive fermentation process

B. Tartakovsky*, B. E. Dainson, D. R. Lewin, M. Sheintuch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Optimal regulatory control of an autoinductive recombinant culture in a fed-batch reactor is considered. End point optimization results in a three-stage process: biomass growth, inducer synthesis and product synthesis. It is shown that in the last stage the substrate concentration should be maintained constant. This is achieved using an input-output linearizing controller accompanied by a novel non-linear state observer for the estimation of unmeasured state variables on the basis of on-line off-gas carbon dioxide concentration measurements. Experimental runs of luminous recombinant E. coli strain in a laboratory fermenter demonstrate the rapid convergence of the observer estimates as well as the effectiveness and robustness of the overall control system.

Original languageEnglish
Pages (from-to)139-148
Number of pages10
JournalChemical Engineering Journal and the Biochemical Engineering Journal
Issue number2
StatePublished - 1996
Externally publishedYes


  • Fed batch autoinductive fermentation process
  • Non-linear control
  • Optimal regulatory control


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