Analysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoir

TY - JOUR

T1 - Analysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoir

AU - Mbadinga, Serge Maurice

AU - Li, Kai Ping

AU - Zhou, Lei

AU - Wang, Li Ying

AU - Yang, Shi Zhong

AU - Liu, Jin Feng

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Funding Information: Acknowledgments This work was supported by the National Natural Science Foundation of China (grant no. 51174092) and the 863 Program (grant no. 2009AA063503).

PY - 2012/10

Y1 - 2012/10

N2 - Microbial assemblage in an n-alkanes-dependent thermophilic methanogenic enrichment cultures derived from production waters of a high-temperature petroleum reservoir was investigated in this study. Substantially higher amounts of methane were generated from the enrichment cultures incubated at 55 °C for 528 days with a mixture of long-chain n-alkanes (C15-C 20). Stoichiometric estimation showed that alkanes-dependent methanogenesis accounted for about 19.8% of the total amount of methane expected. Hydrogen was occasionally detected together with methane in the gas phase of the cultures. Chemical analysis of the liquid cultures resulted only in low concentrations of acetate and formate. Phylogenetic analysis of the enrichment revealed the presence of several bacterial taxa related to Firmicutes, Thermodesulfobiaceae, Thermotogaceae, Nitrospiraceae, Dictyoglomaceae, Candidate division OP8 and others without close cultured representatives, and Archaea predominantly related to uncultured members in the order Archaeoglobales and CO2-reducing methanogens. Screening of genomic DNA retrieved from the alkanesamended enrichment cultures also suggested the presence of new alkylsuccinate synthase alpha-subunit (assA) homologues. These findings suggest the presence of poorly characterized (putative) anaerobic n-alkanes degraders in the thermophilic methanogenic enrichment cultures. Our results indicate that methanogenesis of alkanes under thermophilic condition is likely to proceed via syntrophic acetate and/or formate oxidation linked with hydrogenotrophic methanogenesis.

AB - Microbial assemblage in an n-alkanes-dependent thermophilic methanogenic enrichment cultures derived from production waters of a high-temperature petroleum reservoir was investigated in this study. Substantially higher amounts of methane were generated from the enrichment cultures incubated at 55 °C for 528 days with a mixture of long-chain n-alkanes (C15-C 20). Stoichiometric estimation showed that alkanes-dependent methanogenesis accounted for about 19.8% of the total amount of methane expected. Hydrogen was occasionally detected together with methane in the gas phase of the cultures. Chemical analysis of the liquid cultures resulted only in low concentrations of acetate and formate. Phylogenetic analysis of the enrichment revealed the presence of several bacterial taxa related to Firmicutes, Thermodesulfobiaceae, Thermotogaceae, Nitrospiraceae, Dictyoglomaceae, Candidate division OP8 and others without close cultured representatives, and Archaea predominantly related to uncultured members in the order Archaeoglobales and CO2-reducing methanogens. Screening of genomic DNA retrieved from the alkanesamended enrichment cultures also suggested the presence of new alkylsuccinate synthase alpha-subunit (assA) homologues. These findings suggest the presence of poorly characterized (putative) anaerobic n-alkanes degraders in the thermophilic methanogenic enrichment cultures. Our results indicate that methanogenesis of alkanes under thermophilic condition is likely to proceed via syntrophic acetate and/or formate oxidation linked with hydrogenotrophic methanogenesis.

KW - Alkylsuccinate synthase

KW - High-temperature petroleum reservoir

KW - Hydrogenotrophic methanogenesis

KW - Methanogenic degradation of alkanes

KW - Microbial community

KW - Syntrophic acetate oxidation

UR - http://www.scopus.com/inward/record.url?scp=84866516898&partnerID=8YFLogxK

U2 - 10.1007/s00253-011-3828-8

DO - 10.1007/s00253-011-3828-8

M3 - 文章

C2 - 22249716

AN - SCOPUS:84866516898

SN - 0175-7598

VL - 96

SP - 531

EP - 542

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 2

ER -