TY - JOUR
T1 - Anaerobic Digestion of Dairy Manure in a Fixed Bed CSTR
T2 - Methane Production Performance and Microbial Diversity
AU - Wang, Haipeng
AU - Zhao, Yiquan
AU - Yang, Anyi
AU - Yan, Lei
AU - Lim, Teng Teeh
AU - Zhao, Hongyan
AU - Gu, Ji Dong
AU - Wei, Dan
AU - Wang, Weidong
N1 - Publisher Copyright:
© 2020, North Carolina State University. All rights reserved.
PY - 2020
Y1 - 2020
N2 - To improve the fixed bed and continuous stirred-tank reactor (CSTR) technology relative to the rate of treatment of dairy manure (DM), a continuous stirred tank-treated DM with immobilized carrier biofilm was investigated for 20 days (hydraulic retention time (HRT) was 10 d). Methane productivity, biofilm characteristics, and microbial diversities of the biofilm and digestate were measured. The highest content of extracellular polymeric substances (EPS), proteins, and polysaccharides occurred at 15 days of digestion. An agglomeration phenomenon was observed using a scanning electron microscope on the biofilm. This indicated that the biofilm consisting of EPS was stable during the second HRT. Microbial diversities in digestate and carrier biofilm were characterized using a 16S rRNA sequencing technique. Results showed that the dominant bacterial communities were Pseudomonas (17% to 26%), Clostridium (13% to 21%), and Bacteroidetes (7% to 16%), while the archaea communities were Methanocorpusculum (25% to 37%), Methanosarcina (15% to 33%), Methanoculleus (11% to 15%), and Methanosaeta (13% to 18%). The methane production rate was significantly correlated with bacterial communities (Pseudomonas, Clostridium, Altererythrobacter atlanticus), archaeal communities (Methanosarcina, Methanoculleus, Methanosaeta, and Methanoplanus), and biofilm characteristics (chemical oxygen demand (COD) and EPS). These findings showed that a carrier biofilm could efficiently increase methane production.
AB - To improve the fixed bed and continuous stirred-tank reactor (CSTR) technology relative to the rate of treatment of dairy manure (DM), a continuous stirred tank-treated DM with immobilized carrier biofilm was investigated for 20 days (hydraulic retention time (HRT) was 10 d). Methane productivity, biofilm characteristics, and microbial diversities of the biofilm and digestate were measured. The highest content of extracellular polymeric substances (EPS), proteins, and polysaccharides occurred at 15 days of digestion. An agglomeration phenomenon was observed using a scanning electron microscope on the biofilm. This indicated that the biofilm consisting of EPS was stable during the second HRT. Microbial diversities in digestate and carrier biofilm were characterized using a 16S rRNA sequencing technique. Results showed that the dominant bacterial communities were Pseudomonas (17% to 26%), Clostridium (13% to 21%), and Bacteroidetes (7% to 16%), while the archaea communities were Methanocorpusculum (25% to 37%), Methanosarcina (15% to 33%), Methanoculleus (11% to 15%), and Methanosaeta (13% to 18%). The methane production rate was significantly correlated with bacterial communities (Pseudomonas, Clostridium, Altererythrobacter atlanticus), archaeal communities (Methanosarcina, Methanoculleus, Methanosaeta, and Methanoplanus), and biofilm characteristics (chemical oxygen demand (COD) and EPS). These findings showed that a carrier biofilm could efficiently increase methane production.
KW - Biofilm
KW - Biotechnology
KW - Dairy manure
KW - Fixed bed
KW - Methane
KW - Microbial diversity
UR - http://www.scopus.com/inward/record.url?scp=85120328154&partnerID=8YFLogxK
U2 - 10.15376/biores.15.4.7965-7979
DO - 10.15376/biores.15.4.7965-7979
M3 - 文章
SN - 1930-2126
VL - 15
SP - 7965
EP - 7979
JO - BioResources
JF - BioResources
IS - 4
ER -