The initial soil microbiota impacts the potential for lignocellulose degradation during soil solarization

TY - JOUR

T1 - The initial soil microbiota impacts the potential for lignocellulose degradation during soil solarization

AU - Fernández-Bayo, J. D.

AU - Hestmark, K. V.

AU - Claypool, J. T.

AU - Harrold, D. R.

AU - Randall, T. E.

AU - Achmon, Y.

AU - Stapleton, J. J.

AU - Simmons, C. W.

AU - VanderGheynst, J. S.

N1 - Publisher Copyright: © 2019 The Society for Applied Microbiology

PY - 2019/6

Y1 - 2019/6

N2 - Aims: Soil biosolarization (SBS) is a pest control technology that includes the incorporation of organic matter into soil prior to solarization. The objective of this study was to measure the impact of the initial soil microbiome on the temporal evolution of genes encoding lignocellulose-degrading enzymes during SBS. Methods and Results: Soil biosolarization field experiments were completed using green waste (GW) as a soil amendment and in the presence and absence of compost activating inoculum. Samples were collected over time and at two different soil depths for measurement of the microbial community and the predicted lignocellulosic-degrading microbiome. Compost inoculum had a significant positive effect on several predicted genes encoding enzymes involved in cellulose, hemicellulose and lignin degradation. These included beta-glucosidase, endo-1,3(4)-beta-glucanase, alpha-galactosidase and laccase. Conclusion: Amendment of micro-organisms found in compost to soil prior to SBS enhanced the degradation potential of cellulose, hemicellulose and lignin found in GW. Significance and Impact of the Study: The type of organic matter amended and its biotransformation by soil micro-organisms impact the efficacy of SBS. The results suggest that co-amending highly recalcitrant biomass with micro-organisms found in compost improves biomass conversion during SBS.

AB - Aims: Soil biosolarization (SBS) is a pest control technology that includes the incorporation of organic matter into soil prior to solarization. The objective of this study was to measure the impact of the initial soil microbiome on the temporal evolution of genes encoding lignocellulose-degrading enzymes during SBS. Methods and Results: Soil biosolarization field experiments were completed using green waste (GW) as a soil amendment and in the presence and absence of compost activating inoculum. Samples were collected over time and at two different soil depths for measurement of the microbial community and the predicted lignocellulosic-degrading microbiome. Compost inoculum had a significant positive effect on several predicted genes encoding enzymes involved in cellulose, hemicellulose and lignin degradation. These included beta-glucosidase, endo-1,3(4)-beta-glucanase, alpha-galactosidase and laccase. Conclusion: Amendment of micro-organisms found in compost to soil prior to SBS enhanced the degradation potential of cellulose, hemicellulose and lignin found in GW. Significance and Impact of the Study: The type of organic matter amended and its biotransformation by soil micro-organisms impact the efficacy of SBS. The results suggest that co-amending highly recalcitrant biomass with micro-organisms found in compost improves biomass conversion during SBS.

KW - anaerobic soil disinfestation

KW - biological soil disinfestation

KW - biosolarization

KW - lignocellulose degradation

KW - soil microbiome

KW - soil solarization

UR - http://www.scopus.com/inward/record.url?scp=85065201040&partnerID=8YFLogxK

U2 - 10.1111/jam.14258

DO - 10.1111/jam.14258

M3 - 文章

C2 - 30895681

AN - SCOPUS:85065201040

SN - 1364-5072

VL - 126

SP - 1729

EP - 1741

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 6

ER -