Potassium channel blocker selectively enriched Geobacter from mixed-cultured electroactive biofilm: Insights from microbial community, functional prediction and gene expressions

Qian Zhu; Jingping Hu; Bingchuan Liu; Sha Liang; Keke Xiao; Wenbo Yu; Shushan Yuan; Jiakuan Yang; Huijie Hou

doi:10.1016/j.biortech.2022.128109

Potassium channel blocker selectively enriched Geobacter from mixed-cultured electroactive biofilm: Insights from microbial community, functional prediction and gene expressions

Qian Zhu, Jingping Hu, Bingchuan Liu, Sha Liang, Keke Xiao, Wenbo Yu, Shushan Yuan, Jiakuan Yang, Huijie Hou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This study investigated the effects of electrical signaling disruption induced by adding tetraethylammonium (TEA, a potassium channel blocker) on the formation of mixed-cultured electroactive biofilms, especially the relative abundance of Geobacter over time. Results showed that TEA addition decelerated the biofilm formation, but selectively enriched Geobacter over time (45.8% on Day 32, 67.7% on Day 60 and 78.1% on Day 90), thus resulting in higher final extracellular electron transfer (EET) efficiency. Redundancy analysis (RDA) confirmed that TEA and operation time were significant factors for the selective enrichment of Geobacter. Moreover, increase in cellular processes and signal processing by PICRUSt analysis indicated adaptive responses of electrogenic biofilms to electrical signaling disruption. Furthermore, qRT-PCR indicated the compensatory roles of key cytochromes and pilA in electrochemical communication, which induced Geobacter enrichment. This work provided a broader understanding of electroactive biofilm regulation and potential applications for electricity generation and biosensor in the future.

Original language	English
Article number	128109
Journal	Bioresource Technology
Volume	364
DOIs	https://doi.org/10.1016/j.biortech.2022.128109
State	Published - Nov 2022
Externally published	Yes

Keywords

Geobacter
Microbial community
Mixed-cultured electroactive biofilm
Potassium channel blocker
Selective enrichment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2022.128109

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title = "Potassium channel blocker selectively enriched Geobacter from mixed-cultured electroactive biofilm: Insights from microbial community, functional prediction and gene expressions",

abstract = "This study investigated the effects of electrical signaling disruption induced by adding tetraethylammonium (TEA, a potassium channel blocker) on the formation of mixed-cultured electroactive biofilms, especially the relative abundance of Geobacter over time. Results showed that TEA addition decelerated the biofilm formation, but selectively enriched Geobacter over time (45.8% on Day 32, 67.7% on Day 60 and 78.1% on Day 90), thus resulting in higher final extracellular electron transfer (EET) efficiency. Redundancy analysis (RDA) confirmed that TEA and operation time were significant factors for the selective enrichment of Geobacter. Moreover, increase in cellular processes and signal processing by PICRUSt analysis indicated adaptive responses of electrogenic biofilms to electrical signaling disruption. Furthermore, qRT-PCR indicated the compensatory roles of key cytochromes and pilA in electrochemical communication, which induced Geobacter enrichment. This work provided a broader understanding of electroactive biofilm regulation and potential applications for electricity generation and biosensor in the future.",

keywords = "Geobacter, Microbial community, Mixed-cultured electroactive biofilm, Potassium channel blocker, Selective enrichment",

author = "Qian Zhu and Jingping Hu and Bingchuan Liu and Sha Liang and Keke Xiao and Wenbo Yu and Shushan Yuan and Jiakuan Yang and Huijie Hou",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2022",

month = nov,

doi = "10.1016/j.biortech.2022.128109",

language = "英语",

volume = "364",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Potassium channel blocker selectively enriched Geobacter from mixed-cultured electroactive biofilm

T2 - Insights from microbial community, functional prediction and gene expressions

AU - Zhu, Qian

AU - Hu, Jingping

AU - Liu, Bingchuan

AU - Liang, Sha

AU - Xiao, Keke

AU - Yu, Wenbo

AU - Yuan, Shushan

AU - Yang, Jiakuan

AU - Hou, Huijie

PY - 2022/11

Y1 - 2022/11

N2 - This study investigated the effects of electrical signaling disruption induced by adding tetraethylammonium (TEA, a potassium channel blocker) on the formation of mixed-cultured electroactive biofilms, especially the relative abundance of Geobacter over time. Results showed that TEA addition decelerated the biofilm formation, but selectively enriched Geobacter over time (45.8% on Day 32, 67.7% on Day 60 and 78.1% on Day 90), thus resulting in higher final extracellular electron transfer (EET) efficiency. Redundancy analysis (RDA) confirmed that TEA and operation time were significant factors for the selective enrichment of Geobacter. Moreover, increase in cellular processes and signal processing by PICRUSt analysis indicated adaptive responses of electrogenic biofilms to electrical signaling disruption. Furthermore, qRT-PCR indicated the compensatory roles of key cytochromes and pilA in electrochemical communication, which induced Geobacter enrichment. This work provided a broader understanding of electroactive biofilm regulation and potential applications for electricity generation and biosensor in the future.

AB - This study investigated the effects of electrical signaling disruption induced by adding tetraethylammonium (TEA, a potassium channel blocker) on the formation of mixed-cultured electroactive biofilms, especially the relative abundance of Geobacter over time. Results showed that TEA addition decelerated the biofilm formation, but selectively enriched Geobacter over time (45.8% on Day 32, 67.7% on Day 60 and 78.1% on Day 90), thus resulting in higher final extracellular electron transfer (EET) efficiency. Redundancy analysis (RDA) confirmed that TEA and operation time were significant factors for the selective enrichment of Geobacter. Moreover, increase in cellular processes and signal processing by PICRUSt analysis indicated adaptive responses of electrogenic biofilms to electrical signaling disruption. Furthermore, qRT-PCR indicated the compensatory roles of key cytochromes and pilA in electrochemical communication, which induced Geobacter enrichment. This work provided a broader understanding of electroactive biofilm regulation and potential applications for electricity generation and biosensor in the future.

KW - Geobacter

KW - Microbial community

KW - Mixed-cultured electroactive biofilm

KW - Potassium channel blocker

KW - Selective enrichment

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U2 - 10.1016/j.biortech.2022.128109

DO - 10.1016/j.biortech.2022.128109

M3 - 文章

C2 - 36244602

AN - SCOPUS:85140145089

SN - 0960-8524

VL - 364

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 128109

ER -

Potassium channel blocker selectively enriched Geobacter from mixed-cultured electroactive biofilm: Insights from microbial community, functional prediction and gene expressions

Abstract

Keywords

UN SDGs

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