Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers

Seong Ying Choi; Olivier Habimana; Peter Flood; Emmanuel G. Reynaud; Brian J. Rodriguez; Nan Zhang; Eoin Casey; Michael D. Gilchrist

doi:10.1016/j.colsurfb.2016.04.032

Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers

Seong Ying Choi, Olivier Habimana, Peter Flood, Emmanuel G. Reynaud, Brian J. Rodriguez, Nan Zhang, Eoin Casey, Michael D. Gilchrist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (R_a 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at R_a 0.4 μm surfaces, with material-dependent effects strongest at R_a 0.01 μm. R_a 0.1 μm surfaces exhibited strongest feature-dependent effects and R_a 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at R_a 0.4 μm for both materials. Mammalian cell adhesion is lowest in R_a 0.1 μm and highest in R_a 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.

Original language	English
Pages (from-to)	46-54
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	145
DOIs	https://doi.org/10.1016/j.colsurfb.2016.04.032
State	Published - 1 Sep 2016
Externally published	Yes

Keywords

Cyclic olefin copolymer (COC)
Interface
Madin-Darby Canine Kidney (MDCK) cells
Polymethylmethacrylate (PMMA)
Pseudomonas fluorescens
Surface topography

Access to Document

10.1016/j.colsurfb.2016.04.032

Cite this

@article{ac4e754e05a24891b59e8b026dd7ae19,

title = "Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers",

abstract = "Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4 μm surfaces, with material-dependent effects strongest at Ra 0.01 μm. Ra 0.1 μm surfaces exhibited strongest feature-dependent effects and Ra 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4 μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1 μm and highest in Ra 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.",

keywords = "Cyclic olefin copolymer (COC), Interface, Madin-Darby Canine Kidney (MDCK) cells, Polymethylmethacrylate (PMMA), Pseudomonas fluorescens, Surface topography",

author = "Choi, {Seong Ying} and Olivier Habimana and Peter Flood and Reynaud, {Emmanuel G.} and Rodriguez, {Brian J.} and Nan Zhang and Eoin Casey and Gilchrist, {Michael D.}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = sep,

day = "1",

doi = "10.1016/j.colsurfb.2016.04.032",

language = "英语",

volume = "145",

pages = "46--54",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

}

TY - JOUR

T1 - Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers

AU - Choi, Seong Ying

AU - Habimana, Olivier

AU - Flood, Peter

AU - Reynaud, Emmanuel G.

AU - Rodriguez, Brian J.

AU - Zhang, Nan

AU - Casey, Eoin

AU - Gilchrist, Michael D.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4 μm surfaces, with material-dependent effects strongest at Ra 0.01 μm. Ra 0.1 μm surfaces exhibited strongest feature-dependent effects and Ra 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4 μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1 μm and highest in Ra 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.

AB - Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4 μm surfaces, with material-dependent effects strongest at Ra 0.01 μm. Ra 0.1 μm surfaces exhibited strongest feature-dependent effects and Ra 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4 μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1 μm and highest in Ra 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.

KW - Cyclic olefin copolymer (COC)

KW - Interface

KW - Madin-Darby Canine Kidney (MDCK) cells

KW - Polymethylmethacrylate (PMMA)

KW - Pseudomonas fluorescens

KW - Surface topography

UR - http://www.scopus.com/inward/record.url?scp=84964430196&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2016.04.032

DO - 10.1016/j.colsurfb.2016.04.032

M3 - 文章

C2 - 27137802

AN - SCOPUS:84964430196

SN - 0927-7765

VL - 145

SP - 46

EP - 54

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this