Inhibitory effects of sanguinarine against the cyanobacterium Microcystis aeruginosa NIES-843 and possible mechanisms of action

TY - JOUR

T1 - Inhibitory effects of sanguinarine against the cyanobacterium Microcystis aeruginosa NIES-843 and possible mechanisms of action

AU - Shao, Jihai

AU - Liu, Deming

AU - Gong, Daoxin

AU - Zeng, Qingru

AU - Yan, Zhiyong

AU - Gu, Ji Dong

N1 - Funding Information: The work was supported by the National Natural Science Foundation of China (No. 21107024 ) (J.-H.S.); the Foundation of Furong Scholar Project of Hunan Province, and additionally an honorary professorship (J.-D.G.).

PY - 2013/10/15

Y1 - 2013/10/15

N2 - Sanguinarine showed strong inhibitory effect against Microcystis aeruginosa, a typical water bloom-forming and microcystins-producing cyanobacterium. The EC50 of sanguinarine against the growth of M. aeruginosa NIES-843 was 34.54. =. 1.17. μg/L. Results of chlorophyll fluorescence transient analysis indicated that all the electron donating side, accepting side, and the reaction center of the Photosystem II (PS II) were the targets of sanguinarine against M. aeruginosa NIES-843. The elevation of reactive oxygen species (ROS) level in the cells of M. aeruginosa NIES-843 upon exposure indicated that sanguinarine induced oxidative stress in the active growing cells of M. aeruginosa NIES-843. Further results of gene expression analysis indicated that DNA damage and cell division inhibition were also involved in the inhibitory action mechanism of sanguinarine against M. aeruginosa NIES-843. The inhibitory characteristics of sanguinarine against M. aeruginosa suggest that the ecological- and public health-risks need to be evaluated before its application in cyanobacterial bloom control to avoid devastating events irreversibly.

AB - Sanguinarine showed strong inhibitory effect against Microcystis aeruginosa, a typical water bloom-forming and microcystins-producing cyanobacterium. The EC50 of sanguinarine against the growth of M. aeruginosa NIES-843 was 34.54. =. 1.17. μg/L. Results of chlorophyll fluorescence transient analysis indicated that all the electron donating side, accepting side, and the reaction center of the Photosystem II (PS II) were the targets of sanguinarine against M. aeruginosa NIES-843. The elevation of reactive oxygen species (ROS) level in the cells of M. aeruginosa NIES-843 upon exposure indicated that sanguinarine induced oxidative stress in the active growing cells of M. aeruginosa NIES-843. Further results of gene expression analysis indicated that DNA damage and cell division inhibition were also involved in the inhibitory action mechanism of sanguinarine against M. aeruginosa NIES-843. The inhibitory characteristics of sanguinarine against M. aeruginosa suggest that the ecological- and public health-risks need to be evaluated before its application in cyanobacterial bloom control to avoid devastating events irreversibly.

KW - Chlorophyll fluorescence

KW - Gene expression

KW - Growth inhibition

KW - Microcystis aeruginosa

KW - Sanguinarine

UR - http://www.scopus.com/inward/record.url?scp=84884391349&partnerID=8YFLogxK

U2 - 10.1016/j.aquatox.2013.08.019

DO - 10.1016/j.aquatox.2013.08.019

M3 - 文章

C2 - 24060579

AN - SCOPUS:84884391349

SN - 0166-445X

VL - 142-143

SP - 257

EP - 263

JO - Aquatic Toxicology

JF - Aquatic Toxicology

ER -