TY - JOUR
T1 - Higher Abundance of Ammonia Oxidizing Archaea than Ammonia Oxidizing Bacteria and Their Communities in Tibetan Alpine Meadow Soils under Long-term Nitrogen Fertilization
AU - Xiao, Rui
AU - Chen, Binze
AU - Liu, Yongjun
AU - Wang, Chuanpei
AU - Gu, Ji Dong
AU - Feng, Huyuan
AU - Du, Guozhen
AU - Ma, Xiaojun
N1 - Funding Information:
This research was supported by National Natural Foundation of China (Grant No: 40930533), National Basic Research Program (2012CB026105), The Fundamental Research Funds for the Central Universities (lzujbky-2010-50, lzujbky-2012-k19) and The Independent Funds of State Key Laboratory of Cryospheric Science (SKLCS-ZZ-2008-01).
PY - 2014/8
Y1 - 2014/8
N2 - Increasing usage of nitrogen fertilizer for food production has resulted in severely environmental problems of nutrients enrichment. This study aimed to examine the response of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) to a long-term nitrogen fertilization in Tibetan alpine meadow. The abundance and composition of both AOB and AOA were assessed using quantitative real-time PCR, cloning and sequencing techniques based on amoA gene under different fertilization gradient (0, 30, 60, 90, and 120 g m-2 year-1). Our results showed that, abundances of AOA amoA genes (ranging from 1.48 × 109 to 2.00 × 109 copies per gram of dry soil) were significantly higher than those of AOB amoA genes (1.25 × 107 to 2.62 × 108 copies per gram of dry soil) under fertilization scenario. The abundance of AOB amoA genes increased with increasing nitrogen fertilization, whereas fertilization had little effect on AOA abundance. Sequences of clone libraries of the different treatments revealed that AOB communities were dominated by representatives of Cluster 4, constituting 48.94-64.44% in each clone library. Sequences of Clusters 9, 1 and 2 were prevalent in soils under higher fertilization. All archaeal amoA sequences recovered were affiliated with the soil/sediment clade and marine sediment clade, and no significant difference was observed on the community structure among different fertilization treatments. Variations in the AOB community structure and abundance were linked to ammonium-N and soil pH induced by different fertilization treatments. These results showed that the abundance and structure of the AOB community respond to the fertilization gradient, not AOA. © 2014
AB - Increasing usage of nitrogen fertilizer for food production has resulted in severely environmental problems of nutrients enrichment. This study aimed to examine the response of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) to a long-term nitrogen fertilization in Tibetan alpine meadow. The abundance and composition of both AOB and AOA were assessed using quantitative real-time PCR, cloning and sequencing techniques based on amoA gene under different fertilization gradient (0, 30, 60, 90, and 120 g m-2 year-1). Our results showed that, abundances of AOA amoA genes (ranging from 1.48 × 109 to 2.00 × 109 copies per gram of dry soil) were significantly higher than those of AOB amoA genes (1.25 × 107 to 2.62 × 108 copies per gram of dry soil) under fertilization scenario. The abundance of AOB amoA genes increased with increasing nitrogen fertilization, whereas fertilization had little effect on AOA abundance. Sequences of clone libraries of the different treatments revealed that AOB communities were dominated by representatives of Cluster 4, constituting 48.94-64.44% in each clone library. Sequences of Clusters 9, 1 and 2 were prevalent in soils under higher fertilization. All archaeal amoA sequences recovered were affiliated with the soil/sediment clade and marine sediment clade, and no significant difference was observed on the community structure among different fertilization treatments. Variations in the AOB community structure and abundance were linked to ammonium-N and soil pH induced by different fertilization treatments. These results showed that the abundance and structure of the AOB community respond to the fertilization gradient, not AOA. © 2014
KW - Alpine Meadow
KW - ammonia oxidizing archaea
KW - ammonia oxidizing bacteria
KW - nitrification
KW - nitrogen fertilization
UR - http://www.scopus.com/inward/record.url?scp=84904413652&partnerID=8YFLogxK
U2 - 10.1080/01490451.2013.875298
DO - 10.1080/01490451.2013.875298
M3 - 文章
AN - SCOPUS:84904413652
SN - 0149-0451
VL - 31
SP - 597
EP - 604
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 7
ER -
