TY - JOUR
T1 - Genetic insights unraveling quorum quenching potential of indigenous isolates from an anaerobic membrane bioreactor
AU - Waheed, Hira
AU - Mehmood, Ch. Tahir
AU - Li, Yiwei
AU - Yang, Yongyu
AU - Xiao, Yeyuan
PY - 2022/3/10
Y1 - 2022/3/10
N2 - Despite a few reports of quorum quenching (QQ) in anaerobic membrane bioreactors (AnMBRs), the sensing, regulation and degradation mechanism for quorum sensing (QS) signals by indigenous QQ isolates have been barely studied. This study employed isolation and screening of indigenous QQ strains from anaerobic sludge for acyl-homoserine lactones (AHLs) degradation and membrane biofouling control. High-quality whole genome sequences of Micrococcus luteus anQ-m1, Bacillus pacificus anQ-h4, and Lysinibacillus capsici anQ-h6 were obtained, with a genome size of 2.5, 5.6, and 4.7 Mbp, respectively. Amidase-encoding amiE was the only QQ gene in anQ-m1, while anQ-h6 carries both amiE and lactonase-encoding aiiB genes. Genes responsible for QS autoinducer synthesis were not identified in anQ-m1 and anQ-h6, suggesting low potential of biofilm promotion via QS. Despite a peptidic QS system responsible for biofilm formation, anQ-h4 bears the most comprehensive QQ system, including amiE-amidase, aiiA-lactonase, CYP102A5-cytochrome oxidoreductase, and lsrK-autoinducer-2 kinase. This study elucidates QS and QQ mechanisms of potential anaerobes and provides fundamentals for designing QQ consortia to effectively control biofouling in AnMBRs.
AB - Despite a few reports of quorum quenching (QQ) in anaerobic membrane bioreactors (AnMBRs), the sensing, regulation and degradation mechanism for quorum sensing (QS) signals by indigenous QQ isolates have been barely studied. This study employed isolation and screening of indigenous QQ strains from anaerobic sludge for acyl-homoserine lactones (AHLs) degradation and membrane biofouling control. High-quality whole genome sequences of Micrococcus luteus anQ-m1, Bacillus pacificus anQ-h4, and Lysinibacillus capsici anQ-h6 were obtained, with a genome size of 2.5, 5.6, and 4.7 Mbp, respectively. Amidase-encoding amiE was the only QQ gene in anQ-m1, while anQ-h6 carries both amiE and lactonase-encoding aiiB genes. Genes responsible for QS autoinducer synthesis were not identified in anQ-m1 and anQ-h6, suggesting low potential of biofilm promotion via QS. Despite a peptidic QS system responsible for biofilm formation, anQ-h4 bears the most comprehensive QQ system, including amiE-amidase, aiiA-lactonase, CYP102A5-cytochrome oxidoreductase, and lsrK-autoinducer-2 kinase. This study elucidates QS and QQ mechanisms of potential anaerobes and provides fundamentals for designing QQ consortia to effectively control biofouling in AnMBRs.
KW - Biofouling control
KW - Anaerobic quorum quenching
KW - Whole-genome sequencing
KW - Micrococcus luteus
KW - Bacillus pacificus
KW - Lysinibacillus capsici
U2 - 10.1016/j.scitotenv.2021.152349
DO - 10.1016/j.scitotenv.2021.152349
M3 - 文章
SN - 0048-9697
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -