Engineering synergetic CO₂-fixing pathways for malate production

TY - JOUR

T1 - Engineering synergetic CO2-fixing pathways for malate production

AU - Hu, Guipeng

AU - Zhou, Jie

AU - Chen, Xiulai

AU - Qian, Yuanyuan

AU - Gao, Cong

AU - Guo, Liang

AU - Xu, Peng

AU - Chen, Wei

AU - Chen, Jian

AU - Li, Yin

AU - Liu, Liming

N1 - Publisher Copyright: © 2018

PY - 2018/5

Y1 - 2018/5

N2 - Increasing the microbial CO2-fixing efficiency often requires supplying sufficient ATP and redirecting carbon flux for the production of metabolites. However, addressing these two issues concurrently remains a challenge. Here, we present a combinational strategy based on a synergetic CO2-fixing pathway that combines an ATP-generating carboxylation reaction in the central metabolic pathway with the ATP-consuming RuBisCO shunt in the carbon fixation pathway. This strategy provides enough ATP to improve the efficiency of CO2 fixation and simultaneously rewires the CO2-fixing pathway to the central metabolic pathway for the biosynthesis of chemicals. We demonstrate the application of this strategy by increasing the CO2-fixing rate and malate production in the autotroph Synechococcus elongatus by 110% and to 260 μM respectively, as well as increasing these two factors in the heterotrophic CO2-fixing Escherichia coli by 870% and to 387 mM respectively.

AB - Increasing the microbial CO2-fixing efficiency often requires supplying sufficient ATP and redirecting carbon flux for the production of metabolites. However, addressing these two issues concurrently remains a challenge. Here, we present a combinational strategy based on a synergetic CO2-fixing pathway that combines an ATP-generating carboxylation reaction in the central metabolic pathway with the ATP-consuming RuBisCO shunt in the carbon fixation pathway. This strategy provides enough ATP to improve the efficiency of CO2 fixation and simultaneously rewires the CO2-fixing pathway to the central metabolic pathway for the biosynthesis of chemicals. We demonstrate the application of this strategy by increasing the CO2-fixing rate and malate production in the autotroph Synechococcus elongatus by 110% and to 260 μM respectively, as well as increasing these two factors in the heterotrophic CO2-fixing Escherichia coli by 870% and to 387 mM respectively.

KW - ATP balance

KW - CO fixation

KW - Malate production

KW - Pathway engineering

UR - http://www.scopus.com/inward/record.url?scp=85047097558&partnerID=8YFLogxK

U2 - 10.1016/j.ymben.2018.05.007

DO - 10.1016/j.ymben.2018.05.007

M3 - 文章

C2 - 29753840

AN - SCOPUS:85047097558

SN - 1096-7176

VL - 47

SP - 496

EP - 504

JO - Metabolic Engineering

JF - Metabolic Engineering

ER -