Effect of Sodium on Methanogens in a Two-Stage Anaerobic System

Keke Xiao; Yogananda Maspolim; Yan Zhou; Chenghong Guo; Wun Jern Ng

doi:10.3390/app12030956

Effect of Sodium on Methanogens in a Two-Stage Anaerobic System

Keke Xiao, Yogananda Maspolim, Yan Zhou, Chenghong Guo^*, Wun Jern Ng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

This study evaluated the effects of sodium on anaerobic biomass from the second-stage reactor of a two-stage anaerobic digester. The results indicated that methanogens showed a rela-tively high sodium tolerance of 2.4 g Na⁺ L⁻¹ . Microbial community analysis showed that viable Methanomicrobiales was the most abundant population by a combined propidium monoazide cross-linking quantitative polymerase chain reaction technique. There was a population shift towards higher abundance of Thermotoga (0.02%), Clostridium (2.50%) and Methanoculleus (13.80%). Biomass activity in relation to increased sodium concentrations was investigated with the adenosine triphos-phate test coupled with extracellular polymeric substances measurement. The results showed biomass activity decreased from 33 to 16 µg g⁻¹ volatile suspended solids as sodium concentrations increased from 1.3 to 9.1 g Na⁺ L⁻¹ . Higher EPS production, particularly a greater predominance of carbo-hydrates, was stimulated by higher sodium concentrations. This study provides insights into the superiority of sodium tolerance of two-stage anaerobic digester in compared with a single-stage anaerobic system.

Original language	English
Article number	956
Journal	Applied Sciences (Switzerland)
Volume	12
Issue number	3
DOIs	https://doi.org/10.3390/app12030956
State	Published - 1 Feb 2022
Externally published	Yes

Keywords

Biomass activity
Extracellular polymeric substances
Methanogens
Sodium inhibition
Two-stage anaerobic digester

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10.3390/app12030956

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title = "Effect of Sodium on Methanogens in a Two-Stage Anaerobic System",

abstract = "This study evaluated the effects of sodium on anaerobic biomass from the second-stage reactor of a two-stage anaerobic digester. The results indicated that methanogens showed a rela-tively high sodium tolerance of 2.4 g Na+ L−1 . Microbial community analysis showed that viable Methanomicrobiales was the most abundant population by a combined propidium monoazide cross-linking quantitative polymerase chain reaction technique. There was a population shift towards higher abundance of Thermotoga (0.02%), Clostridium (2.50%) and Methanoculleus (13.80%). Biomass activity in relation to increased sodium concentrations was investigated with the adenosine triphos-phate test coupled with extracellular polymeric substances measurement. The results showed biomass activity decreased from 33 to 16 µg g−1 volatile suspended solids as sodium concentrations increased from 1.3 to 9.1 g Na+ L−1 . Higher EPS production, particularly a greater predominance of carbo-hydrates, was stimulated by higher sodium concentrations. This study provides insights into the superiority of sodium tolerance of two-stage anaerobic digester in compared with a single-stage anaerobic system.",

keywords = "Biomass activity, Extracellular polymeric substances, Methanogens, Sodium inhibition, Two-stage anaerobic digester",

author = "Keke Xiao and Yogananda Maspolim and Yan Zhou and Chenghong Guo and Ng, {Wun Jern}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

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doi = "10.3390/app12030956",

language = "英语",

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AU - Xiao, Keke

AU - Maspolim, Yogananda

AU - Zhou, Yan

AU - Guo, Chenghong

AU - Ng, Wun Jern

PY - 2022/2/1

Y1 - 2022/2/1

N2 - This study evaluated the effects of sodium on anaerobic biomass from the second-stage reactor of a two-stage anaerobic digester. The results indicated that methanogens showed a rela-tively high sodium tolerance of 2.4 g Na+ L−1 . Microbial community analysis showed that viable Methanomicrobiales was the most abundant population by a combined propidium monoazide cross-linking quantitative polymerase chain reaction technique. There was a population shift towards higher abundance of Thermotoga (0.02%), Clostridium (2.50%) and Methanoculleus (13.80%). Biomass activity in relation to increased sodium concentrations was investigated with the adenosine triphos-phate test coupled with extracellular polymeric substances measurement. The results showed biomass activity decreased from 33 to 16 µg g−1 volatile suspended solids as sodium concentrations increased from 1.3 to 9.1 g Na+ L−1 . Higher EPS production, particularly a greater predominance of carbo-hydrates, was stimulated by higher sodium concentrations. This study provides insights into the superiority of sodium tolerance of two-stage anaerobic digester in compared with a single-stage anaerobic system.

AB - This study evaluated the effects of sodium on anaerobic biomass from the second-stage reactor of a two-stage anaerobic digester. The results indicated that methanogens showed a rela-tively high sodium tolerance of 2.4 g Na+ L−1 . Microbial community analysis showed that viable Methanomicrobiales was the most abundant population by a combined propidium monoazide cross-linking quantitative polymerase chain reaction technique. There was a population shift towards higher abundance of Thermotoga (0.02%), Clostridium (2.50%) and Methanoculleus (13.80%). Biomass activity in relation to increased sodium concentrations was investigated with the adenosine triphos-phate test coupled with extracellular polymeric substances measurement. The results showed biomass activity decreased from 33 to 16 µg g−1 volatile suspended solids as sodium concentrations increased from 1.3 to 9.1 g Na+ L−1 . Higher EPS production, particularly a greater predominance of carbo-hydrates, was stimulated by higher sodium concentrations. This study provides insights into the superiority of sodium tolerance of two-stage anaerobic digester in compared with a single-stage anaerobic system.

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KW - Extracellular polymeric substances

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KW - Sodium inhibition

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JO - Applied Sciences (Switzerland)

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ER -

Effect of Sodium on Methanogens in a Two-Stage Anaerobic System

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Keywords

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