Comparison of biomass detachment from biofilms of two different Pseudomonas spp. under constant shear conditions

Giulio Gazzola; Olivier Habimana; Cormac D. Murphy; Eoin Casey

doi:10.1080/08927014.2014.990962

Comparison of biomass detachment from biofilms of two different Pseudomonas spp. under constant shear conditions

Giulio Gazzola, Olivier Habimana, Cormac D. Murphy, Eoin Casey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In the context of biofilm development, detachment is of practical importance when placed in a biofilm management perspective. The objective of the present study was to examine biofilm structure and biofilm detachment under controlled conditions for two distinct microorganisms grown under constant shear conditions. Detached biofilm biomass was regularly collected and analysed over the course of 72 h biofilm growth by Pseudomonas putida and Pseudomonas fluorescens cells, and biofilm structural development assessed using confocal microscopy. The two Pseudomonas spp., which had very similar specific growth rates in planktonic culture, presented notably different characteristics in terms of biofilm morphology but their detachment behaviours over time were very similar. These findings underline the intrinsic complexity of the detachment phenomenon.

Original language	English
Pages (from-to)	13-18
Number of pages	6
Journal	Biofouling
Volume	31
Issue number	1
DOIs	https://doi.org/10.1080/08927014.2014.990962
State	Published - 2 Jan 2015
Externally published	Yes

Keywords

Pseudomonas
biofilm
detachment
shear
structure

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10.1080/08927014.2014.990962

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abstract = "In the context of biofilm development, detachment is of practical importance when placed in a biofilm management perspective. The objective of the present study was to examine biofilm structure and biofilm detachment under controlled conditions for two distinct microorganisms grown under constant shear conditions. Detached biofilm biomass was regularly collected and analysed over the course of 72 h biofilm growth by Pseudomonas putida and Pseudomonas fluorescens cells, and biofilm structural development assessed using confocal microscopy. The two Pseudomonas spp., which had very similar specific growth rates in planktonic culture, presented notably different characteristics in terms of biofilm morphology but their detachment behaviours over time were very similar. These findings underline the intrinsic complexity of the detachment phenomenon.",

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AU - Gazzola, Giulio

AU - Habimana, Olivier

AU - Murphy, Cormac D.

AU - Casey, Eoin

PY - 2015/1/2

Y1 - 2015/1/2

N2 - In the context of biofilm development, detachment is of practical importance when placed in a biofilm management perspective. The objective of the present study was to examine biofilm structure and biofilm detachment under controlled conditions for two distinct microorganisms grown under constant shear conditions. Detached biofilm biomass was regularly collected and analysed over the course of 72 h biofilm growth by Pseudomonas putida and Pseudomonas fluorescens cells, and biofilm structural development assessed using confocal microscopy. The two Pseudomonas spp., which had very similar specific growth rates in planktonic culture, presented notably different characteristics in terms of biofilm morphology but their detachment behaviours over time were very similar. These findings underline the intrinsic complexity of the detachment phenomenon.

AB - In the context of biofilm development, detachment is of practical importance when placed in a biofilm management perspective. The objective of the present study was to examine biofilm structure and biofilm detachment under controlled conditions for two distinct microorganisms grown under constant shear conditions. Detached biofilm biomass was regularly collected and analysed over the course of 72 h biofilm growth by Pseudomonas putida and Pseudomonas fluorescens cells, and biofilm structural development assessed using confocal microscopy. The two Pseudomonas spp., which had very similar specific growth rates in planktonic culture, presented notably different characteristics in terms of biofilm morphology but their detachment behaviours over time were very similar. These findings underline the intrinsic complexity of the detachment phenomenon.

KW - Pseudomonas

KW - biofilm

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KW - shear

KW - structure

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Comparison of biomass detachment from biofilms of two different Pseudomonas spp. under constant shear conditions

Abstract

Keywords

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