Bio-consolidation of cracks in masonry cement mortars by Acinetobacter sp. SC4 isolated from a karst cave

TY - JOUR

T1 - Bio-consolidation of cracks in masonry cement mortars by Acinetobacter sp. SC4 isolated from a karst cave

AU - Li, Mengmeng

AU - Fang, Chaolin

AU - Kawasaki, Satoru

AU - Huang, Minsheng

AU - Achal, Varenyam

N1 - Publisher Copyright: © 2018 Elsevier Ltd

PY - 2019/7

Y1 - 2019/7

N2 - Microbially induced carbonate precipitation (MICP)is one of techniques for consolidation of cementitious materials; however, limited biodiversity and inadequate information on its application suggests further extensive research in this area. In this study, bacteria with ability to precipitate carbonate, preferably CaCO3, were isolated from Yixing Shanjuan Cave, China after analyzing bacterial diversity using Illumina MiSeq sequencing the V3-V4 region of 16S rRNA gene. A total of 58,712 effective 16S rRNA gene sequences were obtained, classified into 31 bacterial phyla. One of the highest urease-producing bacterium, Acinetobacter sp. SC4, was then used in consolidation of cracks created in masonry cement mortars. The bio-consolidation led to significant improvement in compressive strength and reduction in water absorption of mortars. It formed calcite observed under scanning electron microscopy, which were in agreement with X-ray diffraction and thermogravimetric analysis. The presented work is the first extensive study on Acinetobacter sp. induced carbonate precipitation in MICP related research.

AB - Microbially induced carbonate precipitation (MICP)is one of techniques for consolidation of cementitious materials; however, limited biodiversity and inadequate information on its application suggests further extensive research in this area. In this study, bacteria with ability to precipitate carbonate, preferably CaCO3, were isolated from Yixing Shanjuan Cave, China after analyzing bacterial diversity using Illumina MiSeq sequencing the V3-V4 region of 16S rRNA gene. A total of 58,712 effective 16S rRNA gene sequences were obtained, classified into 31 bacterial phyla. One of the highest urease-producing bacterium, Acinetobacter sp. SC4, was then used in consolidation of cracks created in masonry cement mortars. The bio-consolidation led to significant improvement in compressive strength and reduction in water absorption of mortars. It formed calcite observed under scanning electron microscopy, which were in agreement with X-ray diffraction and thermogravimetric analysis. The presented work is the first extensive study on Acinetobacter sp. induced carbonate precipitation in MICP related research.

KW - Acinetobacter

KW - Calcite

KW - Consolidation

KW - Mortars

KW - Urease

UR - http://www.scopus.com/inward/record.url?scp=85045245235&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2018.03.008

DO - 10.1016/j.ibiod.2018.03.008

M3 - 文章

AN - SCOPUS:85045245235

SN - 0964-8305

VL - 141

SP - 94

EP - 100

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

ER -