TY - JOUR
T1 - Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems
T2 - Optimization and degradation pathways
AU - Zhuang, Mei
AU - Sanganyado, Edmond
AU - Zhang, Xinxin
AU - Xu, Liang
AU - Zhu, Jianming
AU - Liu, Wenhua
AU - Song, Haihong
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L−1, azo dye concentration: 100–2000 mg L−1) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.
AB - Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L−1, azo dye concentration: 100–2000 mg L−1) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.
KW - Azo dye-degrading bacteria
KW - Azo reductase
KW - Biodegradation
KW - High throughput sequencing
KW - Nearshore sediment
UR - http://www.scopus.com/inward/record.url?scp=85078836891&partnerID=8YFLogxK
U2 - 10.1016/j.jenvman.2020.110222
DO - 10.1016/j.jenvman.2020.110222
M3 - 文章
C2 - 32148291
AN - SCOPUS:85078836891
SN - 0301-4797
VL - 261
JO - Journal of Environmental Management
JF - Journal of Environmental Management
M1 - 110222
ER -