Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri

Tian Tian Liang; Lei Zhou; Muhammad Irfan; Yang Bai; Xue Zhi Liu; Ji Liang Zhang; Zong Yang Wu; Wen Zhuo Wang; Jin Feng Liu; Lei Cheng; Shi Zhong Yang; Ru Qiang Ye; Ji Dong Gu; Bo Zhong Mu

doi:10.1002/celc.202000918

Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri

Tian Tian Liang, Lei Zhou, Muhammad Irfan, Yang Bai, Xue Zhi Liu, Ji Liang Zhang, Zong Yang Wu, Wen Zhuo Wang, Jin Feng Liu, Lei Cheng, Shi Zhong Yang, Ru Qiang Ye, Ji Dong Gu, Bo Zhong Mu^*

^*Corresponding author for this work

Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Electron-shuttling molecules (ESMs), natural or synthetic, are utilized by microorganisms as electron acceptors or donors to facilitate electron transfer. In this study, five ESMs, namely anthraquinone-2-carboxylic acid (AQC), 9,10-anthraquinone-2-sulfonicacid (AQS), 9,10-anthraquinone-2,6-disulfonic acid (AQDS), Neutral red, and Thionin, were selected to assess their effects on electromethanogenesis under different electrochemical potentials and concentrations. Results showed that the rate of methanogenesis achieved in the presence of AQC was 7.4 times higher than that of the control (without ESMs) at −850 mV (vs. Ag/AgCl). The effectiveness on methanogenesis followed AQC, Neutral Red, AQS, AQDS, and Thionin in a decreasing order. Compared with the control, the addition of ESMs did not affect the biomass of methanogens significantly. Meanwhile, qPCR analysis of the ccdA gene indicated that the abundance of the Cytochrome c gene and the extent of CO₂ reduction to methane correlated positively. The promoting effect of the selective ESMs on methanogenesis was mainly related to their electron transfer capabilities.

Original language	English
Pages (from-to)	3783-3789
Number of pages	7
Journal	ChemElectroChem
Volume	7
Issue number	18
DOIs	https://doi.org/10.1002/celc.202000918
State	Published - 15 Sep 2020

Keywords

AQC
CO
CO storage
ccdA gene
electromethanogenesis
electron shuttling molecules

Access to Document

10.1002/celc.202000918

Cite this

Liang, T. T., Zhou, L., Irfan, M., Bai, Y., Liu, X. Z., Zhang, J. L., Wu, Z. Y., Wang, W. Z., Liu, J. F., Cheng, L., Yang, S. Z., Ye, R. Q., Gu, J. D., & Mu, B. Z. (2020). Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri. ChemElectroChem, 7(18), 3783-3789. https://doi.org/10.1002/celc.202000918

@article{1dc962d79d4142d39db3021c1f19314d,

title = "Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri",

abstract = "Electron-shuttling molecules (ESMs), natural or synthetic, are utilized by microorganisms as electron acceptors or donors to facilitate electron transfer. In this study, five ESMs, namely anthraquinone-2-carboxylic acid (AQC), 9,10-anthraquinone-2-sulfonicacid (AQS), 9,10-anthraquinone-2,6-disulfonic acid (AQDS), Neutral red, and Thionin, were selected to assess their effects on electromethanogenesis under different electrochemical potentials and concentrations. Results showed that the rate of methanogenesis achieved in the presence of AQC was 7.4 times higher than that of the control (without ESMs) at −850 mV (vs. Ag/AgCl). The effectiveness on methanogenesis followed AQC, Neutral Red, AQS, AQDS, and Thionin in a decreasing order. Compared with the control, the addition of ESMs did not affect the biomass of methanogens significantly. Meanwhile, qPCR analysis of the ccdA gene indicated that the abundance of the Cytochrome c gene and the extent of CO2 reduction to methane correlated positively. The promoting effect of the selective ESMs on methanogenesis was mainly related to their electron transfer capabilities.",

keywords = "AQC, CO, CO storage, ccdA gene, electromethanogenesis, electron shuttling molecules",

author = "Liang, {Tian Tian} and Lei Zhou and Muhammad Irfan and Yang Bai and Liu, {Xue Zhi} and Zhang, {Ji Liang} and Wu, {Zong Yang} and Wang, {Wen Zhuo} and Liu, {Jin Feng} and Lei Cheng and Yang, {Shi Zhong} and Ye, {Ru Qiang} and Gu, {Ji Dong} and Mu, {Bo Zhong}",

note = "Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",

year = "2020",

month = sep,

day = "15",

doi = "10.1002/celc.202000918",

language = "英语",

volume = "7",

pages = "3783--3789",

journal = "ChemElectroChem",

issn = "2196-0216",

publisher = "John Wiley and Sons Ltd",

number = "18",

}

TY - JOUR

T1 - Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri

AU - Liang, Tian Tian

AU - Zhou, Lei

AU - Irfan, Muhammad

AU - Bai, Yang

AU - Liu, Xue Zhi

AU - Zhang, Ji Liang

AU - Wu, Zong Yang

AU - Wang, Wen Zhuo

AU - Liu, Jin Feng

AU - Cheng, Lei

AU - Yang, Shi Zhong

AU - Ye, Ru Qiang

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

PY - 2020/9/15

Y1 - 2020/9/15

N2 - Electron-shuttling molecules (ESMs), natural or synthetic, are utilized by microorganisms as electron acceptors or donors to facilitate electron transfer. In this study, five ESMs, namely anthraquinone-2-carboxylic acid (AQC), 9,10-anthraquinone-2-sulfonicacid (AQS), 9,10-anthraquinone-2,6-disulfonic acid (AQDS), Neutral red, and Thionin, were selected to assess their effects on electromethanogenesis under different electrochemical potentials and concentrations. Results showed that the rate of methanogenesis achieved in the presence of AQC was 7.4 times higher than that of the control (without ESMs) at −850 mV (vs. Ag/AgCl). The effectiveness on methanogenesis followed AQC, Neutral Red, AQS, AQDS, and Thionin in a decreasing order. Compared with the control, the addition of ESMs did not affect the biomass of methanogens significantly. Meanwhile, qPCR analysis of the ccdA gene indicated that the abundance of the Cytochrome c gene and the extent of CO2 reduction to methane correlated positively. The promoting effect of the selective ESMs on methanogenesis was mainly related to their electron transfer capabilities.

AB - Electron-shuttling molecules (ESMs), natural or synthetic, are utilized by microorganisms as electron acceptors or donors to facilitate electron transfer. In this study, five ESMs, namely anthraquinone-2-carboxylic acid (AQC), 9,10-anthraquinone-2-sulfonicacid (AQS), 9,10-anthraquinone-2,6-disulfonic acid (AQDS), Neutral red, and Thionin, were selected to assess their effects on electromethanogenesis under different electrochemical potentials and concentrations. Results showed that the rate of methanogenesis achieved in the presence of AQC was 7.4 times higher than that of the control (without ESMs) at −850 mV (vs. Ag/AgCl). The effectiveness on methanogenesis followed AQC, Neutral Red, AQS, AQDS, and Thionin in a decreasing order. Compared with the control, the addition of ESMs did not affect the biomass of methanogens significantly. Meanwhile, qPCR analysis of the ccdA gene indicated that the abundance of the Cytochrome c gene and the extent of CO2 reduction to methane correlated positively. The promoting effect of the selective ESMs on methanogenesis was mainly related to their electron transfer capabilities.

KW - AQC

KW - CO

KW - CO storage

KW - ccdA gene

KW - electromethanogenesis

KW - electron shuttling molecules

UR - http://www.scopus.com/inward/record.url?scp=85091850995&partnerID=8YFLogxK

U2 - 10.1002/celc.202000918

DO - 10.1002/celc.202000918

M3 - 文章

AN - SCOPUS:85091850995

SN - 2196-0216

VL - 7

SP - 3783

EP - 3789

JO - ChemElectroChem

JF - ChemElectroChem

IS - 18

ER -

Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this