Anthraquinone-2,6-disulfonate enhanced biodegradation of dibutyl phthalate: Reducing membrane damage and oxidative stress in bacterial degradation

TY - JOUR

T1 - Anthraquinone-2,6-disulfonate enhanced biodegradation of dibutyl phthalate

T2 - Reducing membrane damage and oxidative stress in bacterial degradation

AU - Zhang, Ying

AU - Shi, Hongtao

AU - Gu, Jidong

AU - Jiao, Yaqi

AU - Han, Siyue

AU - Akindolie, Modupe Sarah

AU - Wang, Yifan

AU - Zhang, Lin

AU - Tao, Yue

N1 - Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/4

Y1 - 2020/4

N2 - Plasticizer dibutyl phthalate (DBP) pollution has received more and more attention. In this study, a DBP degrading bacteria Enterobacter sp. DNB-S2 was found to suffer membrane damage and oxidative stress during DBP degradation. Physiological and transcriptome analysis showed that 100 μmol L−1 anthraquinone-2,6-disulfonate (AQDS) could enhance the ability of strain DNB-S2 for biodegradation of DBP. AQDS adjusted the cell surface structure, including increase levels of hydrophobic and unsaturated fatty acids. These changes increased the chemotactic ability of the strain DNB-S2 to the hydrophobic pollutant DBP and the fluidity of the cell membrane. The expression of methyl chemotactic protein and genes associated with cell membrane-fixed components were up-regulated. AQDS also improved the scavenging ability of ·OH and H2O2 of DNB-S2 by promoting expression genes related to glutathione metabolism, thereby reducing oxidative stress. These results will provide new insights into the biodegradation of DBP.

AB - Plasticizer dibutyl phthalate (DBP) pollution has received more and more attention. In this study, a DBP degrading bacteria Enterobacter sp. DNB-S2 was found to suffer membrane damage and oxidative stress during DBP degradation. Physiological and transcriptome analysis showed that 100 μmol L−1 anthraquinone-2,6-disulfonate (AQDS) could enhance the ability of strain DNB-S2 for biodegradation of DBP. AQDS adjusted the cell surface structure, including increase levels of hydrophobic and unsaturated fatty acids. These changes increased the chemotactic ability of the strain DNB-S2 to the hydrophobic pollutant DBP and the fluidity of the cell membrane. The expression of methyl chemotactic protein and genes associated with cell membrane-fixed components were up-regulated. AQDS also improved the scavenging ability of ·OH and H2O2 of DNB-S2 by promoting expression genes related to glutathione metabolism, thereby reducing oxidative stress. These results will provide new insights into the biodegradation of DBP.

KW - Anthraquinone-2,6-disulfonate

KW - Biodegradation

KW - Cell membrane

KW - Dibutyl phthalate

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=85078236329&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2020.122845

DO - 10.1016/j.biortech.2020.122845

M3 - 文章

C2 - 32000129

AN - SCOPUS:85078236329

SN - 0960-8524

VL - 302

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 122845

ER -