Ureolytic bacteria from electronic waste area, their biological robustness against potentially toxic elements and underlying mechanisms

TY - JOUR

T1 - Ureolytic bacteria from electronic waste area, their biological robustness against potentially toxic elements and underlying mechanisms

AU - Li, Weila

AU - Fishman, Ayelet

AU - Achal, Varenyam

N1 - Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/7/1

Y1 - 2021/7/1

N2 - Ureolytic bacteria can be a promising mediator used for the immobilization of potentially toxic elements via microbially-induced carbonate precipitation (MICP) process from biodegradable ions to carbonate form. Electronic waste (E-waste) environment is very complex compared to general metal contaminated soil, however, MICP has not been studied under such an environment. In this study, three bacterial strains were successfully isolated from an E-waste area in Guiyu, China, and indicated to have positive ureolytic behavior with significant heavy metal resistance (specific to Cu and Pb), among which, a strain of Lysinibacillus sp. was proven to show a great persistence in heavy metal immobilization. This featured strain can tolerate up to 100 ppm copper and 1000 ppm lead according to minimal inhibitory concentration (MIC) results, and its urease activity was well-adapted to metal effects. Results also revealed the positive correlation (R2 = 0.9819) between metal concentrations and surface layer protein content present in bacterial cells. The underlying mechanism on the role of S-layer protein in heavy metal immobilization during biocalcification was elucidated. The metabolic system of heavy metal resistance for these E-waste derived isolates is novel and represents a point of interest for possible environmental applications to immobilize toxic heavy metals from electronic waste sites.

AB - Ureolytic bacteria can be a promising mediator used for the immobilization of potentially toxic elements via microbially-induced carbonate precipitation (MICP) process from biodegradable ions to carbonate form. Electronic waste (E-waste) environment is very complex compared to general metal contaminated soil, however, MICP has not been studied under such an environment. In this study, three bacterial strains were successfully isolated from an E-waste area in Guiyu, China, and indicated to have positive ureolytic behavior with significant heavy metal resistance (specific to Cu and Pb), among which, a strain of Lysinibacillus sp. was proven to show a great persistence in heavy metal immobilization. This featured strain can tolerate up to 100 ppm copper and 1000 ppm lead according to minimal inhibitory concentration (MIC) results, and its urease activity was well-adapted to metal effects. Results also revealed the positive correlation (R2 = 0.9819) between metal concentrations and surface layer protein content present in bacterial cells. The underlying mechanism on the role of S-layer protein in heavy metal immobilization during biocalcification was elucidated. The metabolic system of heavy metal resistance for these E-waste derived isolates is novel and represents a point of interest for possible environmental applications to immobilize toxic heavy metals from electronic waste sites.

KW - Bioremediation

KW - Lysinibacillus sp

KW - Microbial carbonate

KW - S-layer protein

KW - Urease

UR - http://www.scopus.com/inward/record.url?scp=85103699042&partnerID=8YFLogxK

U2 - 10.1016/j.jenvman.2021.112517

DO - 10.1016/j.jenvman.2021.112517

M3 - 文章

C2 - 33836437

AN - SCOPUS:85103699042

SN - 0301-4797

VL - 289

JO - Journal of Environmental Management

JF - Journal of Environmental Management

M1 - 112517

ER -