Ultrastructural Study of the in Vitro Interaction between Biomphalaria glabrata Hemocytes and Schistosoma mansoni Miracidia

Wolfan Araque; Emilia E. Barrios; Pedro Rodríguez; Víctor S. Delgado; Héctor J. Finol

doi:10.1590/S0074-02762003000700008

Ultrastructural Study of the in Vitro Interaction between Biomphalaria glabrata Hemocytes and Schistosoma mansoni Miracidia

Wolfan Araque^*, Emilia E. Barrios, Pedro Rodríguez, Víctor S. Delgado, Héctor J. Finol

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.

Original language	English
Pages (from-to)	905-908
Number of pages	4
Journal	Memorias do Instituto Oswaldo Cruz
Volume	98
Issue number	7
DOIs	https://doi.org/10.1590/S0074-02762003000700008
State	Published - Oct 2003
Externally published	Yes

Keywords

Biomphalaria glabrata
Freeze-fracture replica
Schistosoma mansoni

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10.1590/S0074-02762003000700008

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title = "Ultrastructural Study of the in Vitro Interaction between Biomphalaria glabrata Hemocytes and Schistosoma mansoni Miracidia",

abstract = "Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.",

keywords = "Biomphalaria glabrata, Freeze-fracture replica, Schistosoma mansoni",

author = "Wolfan Araque and Barrios, {Emilia E.} and Pedro Rodr{\'i}guez and Delgado, {V{\'i}ctor S.} and Finol, {H{\'e}ctor J.}",

year = "2003",

month = oct,

doi = "10.1590/S0074-02762003000700008",

language = "英语",

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AU - Araque, Wolfan

AU - Barrios, Emilia E.

AU - Rodríguez, Pedro

AU - Delgado, Víctor S.

AU - Finol, Héctor J.

PY - 2003/10

Y1 - 2003/10

N2 - Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.

AB - Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.

KW - Biomphalaria glabrata

KW - Freeze-fracture replica

KW - Schistosoma mansoni

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Ultrastructural Study of the in Vitro Interaction between Biomphalaria glabrata Hemocytes and Schistosoma mansoni Miracidia

Abstract

Keywords

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