TY - JOUR
T1 - Two Novel Algicidal Isolates Kill Chlorella pyrenoidosa by Inhibiting their Host Antioxidase Activities
AU - Liao, Chunli
AU - Liu, Xiaobo
AU - Liu, Ruifang
AU - Shan, Linna
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/9/28
Y1 - 2015/9/28
N2 - In the biocontrol of harmful algal blooms, there has been considerable interest about the role of algicidal bacteria in algicidal activity. In this experiment, two novel algicidal bacteria (strains NP23 and AM11) against Chlorella pyrenoidosa were isolated from the Baiguishan reservoir in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains NP23 and AM11 belonged to Enterobacter cloacae and Gibberella moniliformis, respectively. To further understand the algicidal activities, five parameters including the chlorophyll a content, cell survival rate, superoxide dismutase (SOD) peroxide dismutase (POD), and catalase (CAT) were tested in the C. pyrenoidosa cells after inoculation with the algicidal bacteria Enterobacter cloacae NP23 and Gibberella moniliformis AM11. As a result, the growth of the treated C. pyrenoidosa was significantly restrained with a great decline of chlorophyll a content. Meanwhile, three antioxidase activities of the treated C. pyrenoidosa were initially stimulated from day 1 to day 3 but then dramatically inhibited at low level. These results induced that the oxidative imbalance (i.e., inhibition of antioxidase activities) caused by algicidal bacteria could be the killing agent of the C. pyrenoidosa cells.
AB - In the biocontrol of harmful algal blooms, there has been considerable interest about the role of algicidal bacteria in algicidal activity. In this experiment, two novel algicidal bacteria (strains NP23 and AM11) against Chlorella pyrenoidosa were isolated from the Baiguishan reservoir in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains NP23 and AM11 belonged to Enterobacter cloacae and Gibberella moniliformis, respectively. To further understand the algicidal activities, five parameters including the chlorophyll a content, cell survival rate, superoxide dismutase (SOD) peroxide dismutase (POD), and catalase (CAT) were tested in the C. pyrenoidosa cells after inoculation with the algicidal bacteria Enterobacter cloacae NP23 and Gibberella moniliformis AM11. As a result, the growth of the treated C. pyrenoidosa was significantly restrained with a great decline of chlorophyll a content. Meanwhile, three antioxidase activities of the treated C. pyrenoidosa were initially stimulated from day 1 to day 3 but then dramatically inhibited at low level. These results induced that the oxidative imbalance (i.e., inhibition of antioxidase activities) caused by algicidal bacteria could be the killing agent of the C. pyrenoidosa cells.
KW - Algal blooms
KW - Algicidal bacteria
KW - Antioxidase activities
KW - Chlorella pyrenoidosa
UR - http://www.scopus.com/inward/record.url?scp=84940447855&partnerID=8YFLogxK
U2 - 10.1007/s12010-015-1749-1
DO - 10.1007/s12010-015-1749-1
M3 - 文章
C2 - 26194712
AN - SCOPUS:84940447855
SN - 0273-2289
VL - 177
SP - 567
EP - 576
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 2
ER -