The large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil

TY - JOUR

T1 - The large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil

AU - Zhu, Xuejiao

AU - Li, Weila

AU - Zhan, Lu

AU - Huang, Minsheng

AU - Zhang, Qiuzhuo

AU - Achal, Varenyam

N1 - Publisher Copyright: © 2016 Elsevier Ltd

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Microbial carbonate precipitation is known as an efficient process for the remediation of heavy metals from contaminated soils. In the present study, a urease positive bacterial isolate, identified as Bacillus cereus NS4 through 16S rDNA sequencing, was utilized on a large scale to remove nickel from industrial soil contaminated by the battery industry. The soil was highly contaminated with an initial total nickel concentration of approximately 900 mg kg−1. The soluble-exchangeable fraction was reduced to 38 mg kg−1 after treatment. The primary objective of metal stabilization was achieved by reducing the bioavailability through immobilizing the nickel in the urease-driven carbonate precipitation. The nickel removal in the soils contributed to the transformation of nickel from mobile species into stable biominerals identified as calcite, vaterite, aragonite and nickelous carbonate when analyzed under XRD. It was proven that during precipitation of calcite, Ni2+ with an ion radius close to Ca2+ was incorporated into the CaCO3 crystal. The biominerals were also characterized by using SEM-EDS to observe the crystal shape and Raman-FTIR spectroscopy to predict responsible bonding during bioremediation with respect to Ni immobilization. The electronic structure and chemical-state information of the detected elements during MICP bioremediation process was studied by XPS. This is the first study in which microbial carbonate precipitation was used for the large-scale remediation of metal-contaminated industrial soil.

AB - Microbial carbonate precipitation is known as an efficient process for the remediation of heavy metals from contaminated soils. In the present study, a urease positive bacterial isolate, identified as Bacillus cereus NS4 through 16S rDNA sequencing, was utilized on a large scale to remove nickel from industrial soil contaminated by the battery industry. The soil was highly contaminated with an initial total nickel concentration of approximately 900 mg kg−1. The soluble-exchangeable fraction was reduced to 38 mg kg−1 after treatment. The primary objective of metal stabilization was achieved by reducing the bioavailability through immobilizing the nickel in the urease-driven carbonate precipitation. The nickel removal in the soils contributed to the transformation of nickel from mobile species into stable biominerals identified as calcite, vaterite, aragonite and nickelous carbonate when analyzed under XRD. It was proven that during precipitation of calcite, Ni2+ with an ion radius close to Ca2+ was incorporated into the CaCO3 crystal. The biominerals were also characterized by using SEM-EDS to observe the crystal shape and Raman-FTIR spectroscopy to predict responsible bonding during bioremediation with respect to Ni immobilization. The electronic structure and chemical-state information of the detected elements during MICP bioremediation process was studied by XPS. This is the first study in which microbial carbonate precipitation was used for the large-scale remediation of metal-contaminated industrial soil.

KW - Biominerals

KW - Calcite

KW - NiCO

KW - Nickel

KW - Remediation

KW - Urease

UR - http://www.scopus.com/inward/record.url?scp=84992724182&partnerID=8YFLogxK

U2 - 10.1016/j.envpol.2016.10.047

DO - 10.1016/j.envpol.2016.10.047

M3 - 文章

C2 - 27814530

AN - SCOPUS:84992724182

SN - 0269-7491

VL - 219

SP - 149

EP - 155

JO - Environmental Pollution

JF - Environmental Pollution

ER -