Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach

TY - JOUR

T1 - Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach

AU - Cao, Huayu

AU - Habimana, Olivier

AU - Safari, Ashkan

AU - Heffernan, Rory

AU - Dai, Yihong

AU - Casey, Eoin

N1 - Publisher Copyright: © 2016 The Author(s).

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Particle-tracking microrheology is an in situ technique that allows quantification of biofilm material properties. It overcomes the limitations of alternative techniques such as bulk rheology or force spectroscopy by providing data on region specific material properties at any required biofilm location and can be combined with confocal microscopy and associated structural analysis. This article describes single particle tracking microrheology combined with confocal laser scanning microscopy to resolve the biofilm structure in 3 dimensions and calculate the creep compliances locally. Samples were analysed from Pseudomonas fluorescens biofilms that were cultivated over two timescales (24 h and 48 h) and alternate ionic conditions (with and without calcium chloride supplementation). The region-based creep compliance analysis showed that the creep compliance of biofilm void zones is the primary contributor to biofilm mechanical properties, contributing to the overall viscoelastic character.

AB - Particle-tracking microrheology is an in situ technique that allows quantification of biofilm material properties. It overcomes the limitations of alternative techniques such as bulk rheology or force spectroscopy by providing data on region specific material properties at any required biofilm location and can be combined with confocal microscopy and associated structural analysis. This article describes single particle tracking microrheology combined with confocal laser scanning microscopy to resolve the biofilm structure in 3 dimensions and calculate the creep compliances locally. Samples were analysed from Pseudomonas fluorescens biofilms that were cultivated over two timescales (24 h and 48 h) and alternate ionic conditions (with and without calcium chloride supplementation). The region-based creep compliance analysis showed that the creep compliance of biofilm void zones is the primary contributor to biofilm mechanical properties, contributing to the overall viscoelastic character.

UR - http://www.scopus.com/inward/record.url?scp=85042176802&partnerID=8YFLogxK

U2 - 10.1038/s41522-016-0005-y

DO - 10.1038/s41522-016-0005-y

M3 - 文章

AN - SCOPUS:85042176802

SN - 2055-5008

VL - 2

JO - npj Biofilms and Microbiomes

JF - npj Biofilms and Microbiomes

IS - 1

M1 - 5

ER -