TY - JOUR
T1 - Remediation of copper-contaminated soil by Kocuria flava CR1, based on microbially induced calcite precipitation
AU - Achal, Varenyam
AU - Pan, Xiangliang
AU - Zhang, Daoyong
N1 - Funding Information:
This work was supported by Knowledge Innovation Program of Chinese Academy of Sciences (KZCX2-YW-335), Program of 100 Distinguished Young Scientists of the Chinese Academy of Sciences, National Natural Science Foundation of China (40673070, 40872169 and 41072195) and Chinese Academy of Sciences Fellowships for Young International Scientists (2010Y2ZB04). We are grateful to the editor and the anonymous reviewers for their valuable comments and suggestions.
PY - 2011/10
Y1 - 2011/10
N2 - An indigenous calcifying bacterial strain CR1, identified as Kocuria flava, was isolated from soil of a mining area, Urumqi, China. An extensive copper bioremediation capacity of this isolate was studied based on microbially induced calcite precipitation (MICP). K. flava CR1 removed 97% of copper when initial Cu concentration was 1000mgL-1. The isolate produced significant amount of urease (472UmL-1), an enzyme that leads to calcite precipitation. The isolate removed 95% of copper from contaminated soil. The MICP process in bioremediation was further confirmed by FTIR and XRD analyses. FTIR analysis showed two different forms of calcium carbonate, i.e., calcite and aragonite, and the results were well supported by XRD. For the first time, the ability of K. flava has been documented in the bioremediation of polluted soil. This study showed that MICP-based bioremediation by K. flava is a viable, environmental friendly technology for cleaning-up the copper-contaminated site.
AB - An indigenous calcifying bacterial strain CR1, identified as Kocuria flava, was isolated from soil of a mining area, Urumqi, China. An extensive copper bioremediation capacity of this isolate was studied based on microbially induced calcite precipitation (MICP). K. flava CR1 removed 97% of copper when initial Cu concentration was 1000mgL-1. The isolate produced significant amount of urease (472UmL-1), an enzyme that leads to calcite precipitation. The isolate removed 95% of copper from contaminated soil. The MICP process in bioremediation was further confirmed by FTIR and XRD analyses. FTIR analysis showed two different forms of calcium carbonate, i.e., calcite and aragonite, and the results were well supported by XRD. For the first time, the ability of K. flava has been documented in the bioremediation of polluted soil. This study showed that MICP-based bioremediation by K. flava is a viable, environmental friendly technology for cleaning-up the copper-contaminated site.
KW - Bioremediation
KW - Calcite
KW - Copper
KW - FTIR
KW - Kocuria flava
UR - http://www.scopus.com/inward/record.url?scp=79960402402&partnerID=8YFLogxK
U2 - 10.1016/j.ecoleng.2011.06.008
DO - 10.1016/j.ecoleng.2011.06.008
M3 - 文章
AN - SCOPUS:79960402402
SN - 0925-8574
VL - 37
SP - 1601
EP - 1605
JO - Ecological Engineering
JF - Ecological Engineering
IS - 10
ER -