Recent progress in high-throughput droplet screening and sorting for bioanalysis

TY - JOUR

T1 - Recent progress in high-throughput droplet screening and sorting for bioanalysis

AU - Sun, Guoyun

AU - Qu, Lisha

AU - Azi, Fidelis

AU - Liu, Yanfeng

AU - Li, Jianghua

AU - Lv, Xueqin

AU - Du, Guocheng

AU - Chen, Jian

AU - Chen, Chia-Hung

AU - Liu, Long

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Owing to its ability to isolate single cells and perform high-throughput sorting, droplet sorting has been widely applied in several research fields. Compared with flow cytometry, droplet allows the encapsulation of single cells for cell secretion or lysate analysis. With the rapid development of this technology in the past decade, various droplet sorting devices with high throughput and accuracy have been developed. A droplet sorter with the highest sorting throughput of 30,000 droplets per second was developed in 2015. Since then, increased attention has been paid to expanding the possibilities of droplet sorting technology and strengthening its advantages over flow cytometry. This review aimed to summarize the recent progress in droplet sorting technology from the perspectives of device design, detection signal, actuating force, and applications. Technical details for improving droplet sorting through various approaches are introduced and discussed. Finally, we discuss the current limitations of droplet sorting for single-cell studies along with the existing gap between the laboratory and industry and provide our insights for future development of droplet sorters.

AB - Owing to its ability to isolate single cells and perform high-throughput sorting, droplet sorting has been widely applied in several research fields. Compared with flow cytometry, droplet allows the encapsulation of single cells for cell secretion or lysate analysis. With the rapid development of this technology in the past decade, various droplet sorting devices with high throughput and accuracy have been developed. A droplet sorter with the highest sorting throughput of 30,000 droplets per second was developed in 2015. Since then, increased attention has been paid to expanding the possibilities of droplet sorting technology and strengthening its advantages over flow cytometry. This review aimed to summarize the recent progress in droplet sorting technology from the perspectives of device design, detection signal, actuating force, and applications. Technical details for improving droplet sorting through various approaches are introduced and discussed. Finally, we discuss the current limitations of droplet sorting for single-cell studies along with the existing gap between the laboratory and industry and provide our insights for future development of droplet sorters.

KW - Droplet sorting

KW - Microfluidic device design

KW - Droplet detecting

KW - Droplet actuating

KW - Synthetic biology

U2 - 10.1016/j.bios.2023.115107

DO - 10.1016/j.bios.2023.115107

M3 - 文章

SN - 0956-5663

VL - 225

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -