Rapid identification of betacyanins from Amaranthus tricolor, Gomphrena globosa, and Hylocereus polyrhizus by matrix-assisted laser desorption/ ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS)

Yi Zhong Cai, Jie Xing, Mei Sun, Harold Corke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Natural betacyanins have attracted great attention as food colorants and potential antioxidants. Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) is a new and powerful technique for the identification of low molecular weight compounds. This study is the first to employ MALDI-QIT-TOF MS to rapidly identify, within a few minutes, a great number of betacyanins in crude extracts from Amaranthus tricolor seedlings, Gomphrena globosa flowers, and Hylocereus polyrhizus fruits. The fresh crude extract samples without any purification were directly used for MALDI-QIT-TOF MS analysis with 2,5-dihydroxybenzoic acid as a matrix. The MS 2 and MS 3 spectrometric data acquired could provide important characteristic information for structural elucidation of the betacyanins. Fourteen free and acylated betacyanins, belonging to amaranthin-type, betanin-type, and gomphrenin-type betacyanins, respectively, were identified. However, the related isomers should be differentiated with the aid of HPLC.

Original languageEnglish
Pages (from-to)6520-6526
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number18
DOIs
StatePublished - 6 Sep 2006
Externally publishedYes

Keywords

  • Amaranth
  • Amaranthus tricolor, gomphrena globosa
  • Betacyanins
  • Betalains
  • Food colorants
  • HPLC
  • Hylocereus polyrhizus
  • Low molecular weight compounds
  • MALDI-QIT-TOF MS
  • Pitaya

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