PTA wastewater molecular toxicity detected with gene chip

Cheng Jun Zhu, Shu Pei Cheng*, Xu Xiang Zhang, You Zhe Lang, Shi Lei Sun, Ji Dong Gu, Da Yong Zhao, Wen Yang Pan, Hong Xia Yu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The purified terephthalic acid (PTA) petrochemical wastewater molecular toxicity detected by use of Mouse Genome 430A 2.0 GeneChip was conducted in this research. The toxic dose to male mice was 0.03 g/(kg·d) of PTA in the wastewater. The mice liver total RNA was isolated as the temple for synthesis of cDNA and then the cDNA as the temple for synthesis of cRNA. Hybridizing the cRNA with the target genes on the gene chip, there were 232 genes expression levels up-regulated and 74 genes down-regulated discovered obviously. The foremost 40 genes for both the highest and the lowest expression levels involved endogenetic steroid and hormone metabolism, immune system, the leukocyte activity and inflammation, detoxification in liver, reproduction and growth hormone, regulation immune factors of anti-tumor and anti-infection and cancer to the mice sampled. The data suggest the PTA wastewater contained over 5 aromatics and their toxicities integrated were much higher than the pure chemical PTA. And the pure chemical PTA toxicities data cannot be used to evaluate the toxicity of the PTA wastewater instead.

Original languageEnglish
Pages (from-to)514-518
Number of pages5
JournalJournal of Environmental Sciences
Issue number3
StatePublished - 2006
Externally publishedYes


  • Gene chip
  • Male mice
  • Molecular toxicity
  • PTA wastewater
  • Predication of security


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