Proposal of possible pathway of fluorene biodegradation by citrobacter sp. fl5

Xuejiao Zhu, Minsheng Huang, Qiuzhuo Zhang, Varenyam Achal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The biodegradation ability of Citrobacter sp. FL5 on fluorene was investigated in the present study. The bacterial isolate was identified based on biochemical test, physiological and 16S rDNA sequence analysis. Fluorene biode-gradation was studied in the liquid media at the initial concentration of 50 mg L−1 fluorene at different pH values (6, 7, 8 and 9) and temperatures (25, 30, 37 and 45°C). Citrobacter sp. FL5 showed maximum performance of fluorene degradation ability at pH 7 and 30°C where it degrades 98% fluorene in liquid media. Furthermore, the isolate degrades 97.5% and 96% of 100 and 150 mg L–1 fluorene respectively at 168 hrs. The possible metabolic pathway for fluorene biodegradation by Citrobacter sp. FL5 was deduced by identification of metabolites through Gas Chromatography Mass Spectrometry (GC-MS) technique. 5 types of metabolites that were detected and identified were dibenzofuran, 9-fluorenylmethanol, methyl benzilate, piperonylic acid and catechol. This study was the first to report and describe the ability of Citrobacter sp. in detail for degradation of higher fluorene concentrations at various pH and temperatures ranges.

Original languageEnglish
Pages (from-to)44-51
Number of pages8
JournalApplied Environmental Biotechnology
Issue number1
StatePublished - 2016
Externally publishedYes


  • Citrobacter sp.
  • Degradation
  • Dibenzofuran
  • Fluorene
  • Gas Chromatography Mass Spectrometry (GC-MS)
  • Metabolites


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