Optimization of liquid media and biosafety assessment for algae-lysing bacterium NP23

Chunli Liao*, Xiaobo Liu, Linna Shan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


To control algal bloom caused by nutrient pollution, a wild-type algae-lysing bacterium was isolated from the Baiguishan reservoir in Henan province of China and identified as Enterobacter sp. strain NP23. Algal culture medium was optimized by applying a Placket–Burman design to obtain a high cell concentration of NP23. Three minerals (i.e., 0.6% KNO3, 0.001% MnSO4·H2O, and 0.3% K2HPO4) were found to be independent factors critical for obtaining the highest cell concentration of 1013 CFU/mL, which was 104 times that of the control. In the algae-lysing experiment, the strain exhibited a high lysis rate for the 4 algae test species, namely, Chlorella vulgari, Scenedesmus, Microcystis wesenbergii, and Chlorella pyrenoidosa. Acute toxicity and mutagenicity tests showed that the bacterium NP23 had no toxic and mutagenic effects on fish, even in large doses such as 107 or 109 CFU/mL. Thus, Enterobacter sp. strain NP23 has strong potential application in the microbial algae-lysing project.

Original languageEnglish
Pages (from-to)593-597
Number of pages5
JournalCanadian Journal of Microbiology
Issue number9
StatePublished - 1 Jul 2014
Externally publishedYes


  • Acute toxicity and mutagenicity tests
  • Algae-lysing bacterium
  • Algal bloom
  • Plackett–Burman design


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