One particle, two targets: A combined action of functionalised gold nanoparticles, against Pseudomonas fluorescens biofilms

Olivier Habimana; Michele Zanoni; Stefania Vitale; Tiina O'Neill; Dimitri Scholz; Bin Xu; Eoin Casey

doi:10.1016/j.jcis.2018.05.014

One particle, two targets: A combined action of functionalised gold nanoparticles, against Pseudomonas fluorescens biofilms

Olivier Habimana, Michele Zanoni, Stefania Vitale, Tiina O'Neill, Dimitri Scholz, Bin Xu, Eoin Casey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Attempts to deal with the problem of detrimental biofilms using nanoparticle technologies have generally focussed on exploiting biocidal approaches. However, it is now recognised that biofilm matrix-components may be targets for the disruption or dispersion of biofilms. Here, we show that the functionalization of gold nanoparticles with the enzyme, proteinase-K (PK) led to both biocidal and matrix disruption effects within Pseudomonas fluorescens biofilms and released cells. This study highlights the potential mechanisms underpinning the properties of Proteinase-K functionalized gold nanoparticles. With the emergence of biocide-resistant biofilm-forming organisms, novel nanoparticle strategies may provide the ideal solution for disrupting and inactivating biofilm cells, thereby minimising the use of biocides or antibiotics.

Original language	English
Pages (from-to)	419-428
Number of pages	10
Journal	Journal of Colloid and Interface Science
Volume	526
DOIs	https://doi.org/10.1016/j.jcis.2018.05.014
State	Published - 15 Sep 2018
Externally published	Yes

Keywords

Biofilm
Enzyme
Nanoparticle

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10.1016/j.jcis.2018.05.014

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title = "One particle, two targets: A combined action of functionalised gold nanoparticles, against Pseudomonas fluorescens biofilms",

abstract = "Attempts to deal with the problem of detrimental biofilms using nanoparticle technologies have generally focussed on exploiting biocidal approaches. However, it is now recognised that biofilm matrix-components may be targets for the disruption or dispersion of biofilms. Here, we show that the functionalization of gold nanoparticles with the enzyme, proteinase-K (PK) led to both biocidal and matrix disruption effects within Pseudomonas fluorescens biofilms and released cells. This study highlights the potential mechanisms underpinning the properties of Proteinase-K functionalized gold nanoparticles. With the emergence of biocide-resistant biofilm-forming organisms, novel nanoparticle strategies may provide the ideal solution for disrupting and inactivating biofilm cells, thereby minimising the use of biocides or antibiotics.",

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doi = "10.1016/j.jcis.2018.05.014",

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T2 - A combined action of functionalised gold nanoparticles, against Pseudomonas fluorescens biofilms

AU - Habimana, Olivier

AU - Zanoni, Michele

AU - Vitale, Stefania

AU - O'Neill, Tiina

AU - Scholz, Dimitri

AU - Xu, Bin

AU - Casey, Eoin

PY - 2018/9/15

Y1 - 2018/9/15

N2 - Attempts to deal with the problem of detrimental biofilms using nanoparticle technologies have generally focussed on exploiting biocidal approaches. However, it is now recognised that biofilm matrix-components may be targets for the disruption or dispersion of biofilms. Here, we show that the functionalization of gold nanoparticles with the enzyme, proteinase-K (PK) led to both biocidal and matrix disruption effects within Pseudomonas fluorescens biofilms and released cells. This study highlights the potential mechanisms underpinning the properties of Proteinase-K functionalized gold nanoparticles. With the emergence of biocide-resistant biofilm-forming organisms, novel nanoparticle strategies may provide the ideal solution for disrupting and inactivating biofilm cells, thereby minimising the use of biocides or antibiotics.

AB - Attempts to deal with the problem of detrimental biofilms using nanoparticle technologies have generally focussed on exploiting biocidal approaches. However, it is now recognised that biofilm matrix-components may be targets for the disruption or dispersion of biofilms. Here, we show that the functionalization of gold nanoparticles with the enzyme, proteinase-K (PK) led to both biocidal and matrix disruption effects within Pseudomonas fluorescens biofilms and released cells. This study highlights the potential mechanisms underpinning the properties of Proteinase-K functionalized gold nanoparticles. With the emergence of biocide-resistant biofilm-forming organisms, novel nanoparticle strategies may provide the ideal solution for disrupting and inactivating biofilm cells, thereby minimising the use of biocides or antibiotics.

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KW - Nanoparticle

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ER -

One particle, two targets: A combined action of functionalised gold nanoparticles, against Pseudomonas fluorescens biofilms

Abstract

Keywords

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