Nitrogen and phosphorus removal using fluidized-carriers in a full-scale A2O biofilm system

Keke Xiao, Lu Zhou*, Beiping He, Liang Qian, Shuo Wan, Liping Qu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


A full-scale biofilm system using fluidized-carriers integrated with anaerobic-anoxic–aerobic process (treatment capacity of 3.75 × 105 m3 d−1) was used for municipal wastewater treatment. The results indicated relatively higher removal efficiencies of 86% total nitrogen (TN), 97% ammonium (NH4-N) and 97% total phosphorus (TP) were achieved, with 0.32 mg L−1 TP, 0.81 mg L−1 NH4-N and 8.07 mg L−1 TN in the effluent, which meet the Class A of Discharge standard of pollutants for municipal wastewater treatment plant (GB18918-2002) of China. Microbial results indicated the dominant microorganisms in the suspended sludge were Proteobacteria and Bacteroidetes at phylum level and β-Proteobacter at class level. The dominant microorganism in the biofilm was Proteobacteria at phylum level, with γ-Proteobacter (17.5%), β-Proteobacter (14%) and δ-Proteobacter (13.08%) distributed at class levels. The presence of Proteobacteria and Bacteroidetes in this system may be related with the phosphorus removal. A reddish color biofilm was formed on the surface of fluidized-carriers in the anaerobic tank and showed specific anammox ability, this may be related with the dominance of 0.0278% Planctomycetaceae at family level and 0.0278% Planctomycetales at order level. Besides the denitrification effects, the possible anammox bacteria present in the anaerobic tank might have also contributed to high nitrogen removal efficiency.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalBiochemical Engineering Journal
StatePublished - 15 Nov 2016
Externally publishedYes


  • Anaerobic-anoxic-aerobic process
  • Biofilm system with fluidized-carriers
  • Biological phosphorus and nitrogen removal
  • Full-scale
  • Microbial community
  • Miseq illumina sequencing


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