Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency

TY - JOUR

T1 - Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency

AU - Han, Yongliang

AU - Liao, Qiong

AU - Yu, Yin

AU - Song, Haixing

AU - Liu, Qiang

AU - Rong, Xiangmin

AU - Gu, Jidong

AU - Lepo, Joe Eugene

AU - Guan, Chunyun

AU - Zhang, Zhenhua

N1 - Publisher Copyright: © 2015, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.

PY - 2015/2

Y1 - 2015/2

N2 - Nitrate (NO3 −) can accumulate in high concentrations in plant cell vacuoles if it is not reduced, reutilized or transported into the cytoplasm. Such accumulation of NO3 − in the vacuole occurs when mechanisms for NO3 − assimilation in the cytoplasm are saturated. Moreover, other processes such as efflux across the plasma membrane might affect NO3 − accumulation in the vacuole. These are the main reasons limiting nitrogen use efficiency (NUE) in plants. This study elucidates mechanisms for NO3 − transport from the cytoplasm to vacuoles by the V-proton pump (V-ATPase and V-PPase) and their relationship with different NUE in four Brassica napus genotypes. Pot experiments were conducted in a greenhouse under normal (15.0 mmol L−1) and limited N (7.5 mmol L−1) concentrations of nitrate using B. napus genotypes that demonstrated either high (742 and Xiangyou 15) or low (814 and H8) NUE (g g−1). Specific inhibitors of V-ATPase and V-PPase increased nitrate reductase (NR) activity, resulting in greatly decreased NO3 − in plant tissues. Nitrate reductase activity and NO3 − content correlated more highly to V-PPase activity than they did to V-ATPase activity, and correlation between V-PPase activity and NO3 − content was significantly higher than it was to V-ATPase. Genotypes with high NUE had significantly lower activities of V-ATPase and V-PPase than those with low NUE. In the high-NUE plants, lower activities of V-proton pump underlie mechanisms that result in significantly lower NO3 − content in plant tissues of the high-NUE genotypes than those found in plant tissues of the low-NUE genotypes. Our results show that the tonoplast proton pumps V-PPase and V-ATPase strongly negatively affect NR activity and positively affect NO3 − content. V-PPase contributed more to this regulatory mechanism than did V-ATPase.

AB - Nitrate (NO3 −) can accumulate in high concentrations in plant cell vacuoles if it is not reduced, reutilized or transported into the cytoplasm. Such accumulation of NO3 − in the vacuole occurs when mechanisms for NO3 − assimilation in the cytoplasm are saturated. Moreover, other processes such as efflux across the plasma membrane might affect NO3 − accumulation in the vacuole. These are the main reasons limiting nitrogen use efficiency (NUE) in plants. This study elucidates mechanisms for NO3 − transport from the cytoplasm to vacuoles by the V-proton pump (V-ATPase and V-PPase) and their relationship with different NUE in four Brassica napus genotypes. Pot experiments were conducted in a greenhouse under normal (15.0 mmol L−1) and limited N (7.5 mmol L−1) concentrations of nitrate using B. napus genotypes that demonstrated either high (742 and Xiangyou 15) or low (814 and H8) NUE (g g−1). Specific inhibitors of V-ATPase and V-PPase increased nitrate reductase (NR) activity, resulting in greatly decreased NO3 − in plant tissues. Nitrate reductase activity and NO3 − content correlated more highly to V-PPase activity than they did to V-ATPase activity, and correlation between V-PPase activity and NO3 − content was significantly higher than it was to V-ATPase. Genotypes with high NUE had significantly lower activities of V-ATPase and V-PPase than those with low NUE. In the high-NUE plants, lower activities of V-proton pump underlie mechanisms that result in significantly lower NO3 − content in plant tissues of the high-NUE genotypes than those found in plant tissues of the low-NUE genotypes. Our results show that the tonoplast proton pumps V-PPase and V-ATPase strongly negatively affect NR activity and positively affect NO3 − content. V-PPase contributed more to this regulatory mechanism than did V-ATPase.

KW - N reutilization

KW - N use efficiency

KW - Oilseed rape (Brassica napus)

KW - Proton pumps in the tonoplast

UR - http://www.scopus.com/inward/record.url?scp=84922224270&partnerID=8YFLogxK

U2 - 10.1007/s11738-014-1744-0

DO - 10.1007/s11738-014-1744-0

M3 - 文章

AN - SCOPUS:84922224270

SN - 0137-5881

VL - 37

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

IS - 2

M1 - 42

ER -