Molecular marker assisted selection for improvement of the eating, cooking and sensory quality of rice (Oryza sativa L.)

TY - JOUR

T1 - Molecular marker assisted selection for improvement of the eating, cooking and sensory quality of rice (Oryza sativa L.)

AU - Jin, Liang

AU - Lu, Yan

AU - Shao, Yafang

AU - Zhang, Gan

AU - Xiao, Peng

AU - Shen, Shengquan

AU - Corke, Harold

AU - Bao, Jinsong

N1 - Funding Information: We thank the anonymous reviewers for their constructive comments. Financial support for this research was provided in part by National High Technology Development Project of China (2006AA10Z193), National Natural Science Foundation of China (30300227, 30771327), the Science and Technology Department of Zhejiang Province (2007C32014), International Atomic Energy Agency (12847/R0), and a Hong Kong Research Grants Council GRF award 2006 (7507/06M).

PY - 2010/1

Y1 - 2010/1

N2 - II-32B is a key maintainer line used for hybrid rice breeding in China. However, it is of poor quality for most Chinese consumers because of its high apparent amylose content (AAC), high gelatinization temperature (GT) and non-fragrance. It is well known that the AAC, GT and fragrance traits are largely controlled by the Wx, starch synthase IIa (SSIIa), and fragrance (fgr) genes, respectively. With the aid of functional markers, we improved the quality of II-32B by introgressing the Wx, SSIIa, and fgr genes from Yixiang B, a fragrant maintainer line that has low AAC and low GT. The microsatellite allele (CT)17 of Wx, the contiguous single nucleotide polymorphism TT allele of SSIIa and the 8-bp deletion allele of fgr were transferred to II-32B by two backcrosses and three selfings. Molecular marker assisted selection was applied in the series to select for individuals carrying Wx-(CT)17, SSIIa-TT, and fgr-deletion alleles. According to the marker genotypes, seventeen homozygous lines for Wx-(CT)17, SSIIa-TT, and fgr gene markers were finally selected. The improved II-32B lines were fragrant with reduced AAC and GT.

AB - II-32B is a key maintainer line used for hybrid rice breeding in China. However, it is of poor quality for most Chinese consumers because of its high apparent amylose content (AAC), high gelatinization temperature (GT) and non-fragrance. It is well known that the AAC, GT and fragrance traits are largely controlled by the Wx, starch synthase IIa (SSIIa), and fragrance (fgr) genes, respectively. With the aid of functional markers, we improved the quality of II-32B by introgressing the Wx, SSIIa, and fgr genes from Yixiang B, a fragrant maintainer line that has low AAC and low GT. The microsatellite allele (CT)17 of Wx, the contiguous single nucleotide polymorphism TT allele of SSIIa and the 8-bp deletion allele of fgr were transferred to II-32B by two backcrosses and three selfings. Molecular marker assisted selection was applied in the series to select for individuals carrying Wx-(CT)17, SSIIa-TT, and fgr-deletion alleles. According to the marker genotypes, seventeen homozygous lines for Wx-(CT)17, SSIIa-TT, and fgr gene markers were finally selected. The improved II-32B lines were fragrant with reduced AAC and GT.

KW - Marker assisted selection

KW - Quality

KW - Rice

KW - Wx gene

UR - http://www.scopus.com/inward/record.url?scp=73549107010&partnerID=8YFLogxK

U2 - 10.1016/j.jcs.2009.11.007

DO - 10.1016/j.jcs.2009.11.007

M3 - 文章

AN - SCOPUS:73549107010

SN - 0733-5210

VL - 51

SP - 159

EP - 164

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 1

ER -