Molecular cloning of class III chitinase gene from Avicennia marina and its expression analysis in response to cadmium and lead stress

TY - JOUR

T1 - Molecular cloning of class III chitinase gene from Avicennia marina and its expression analysis in response to cadmium and lead stress

AU - Wang, Li Ying

AU - Wang, You Shao

AU - Zhang, Jing Ping

AU - Gu, Ji Dong

N1 - Publisher Copyright: © 2015, Springer Science+Business Media New York.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Mangrove species have high tolerance to heavy metal pollution. Chitinases have been widely reported as defense proteins in response to heavy metal stress in terrestrial plants. In this study, a full-length cDNA sequence encoding an acidic and basic class III chitinase (AmCHI III) was cloned by using RT-PCR and RACE methods in Avicennia marina. AmCHI III mRNA expression in leaf of A. marina were investigated under Cd, Pb stresses on using real-time quantitative PCR. The deduced AmCHI III protein consists of 302 amino acids, including a signal putative peptide region, and a catalytic domain. Homology modeling of the catalytic domain revealed a typical molecular structure of class III plant chitinases. Results further demonstrated that the regulation of AmCHI III mRNA expression in leaves was strongly dependent on Cd, Pb stresses. AmCHI III mRNA expressions were significantly increased in response to Cd, Pb, and peaked at 7 days Cd-exposure, 7 days Pb-exposure, respectively. AmCHI III mRNA expression exhibited more sensitive to Pb stress than Cd stress. This work was the first time cloing chitinase from A. marina, and it brought evidence on chitinase gene involving in heavy metals (Cd2+ and Pb2+) resistance or detoxification in plants. Further studies including the promoter and upstream regulation, gene over-expression and the response of mangrove chitinases to other stresses will shed more light on the role of chitinase in mangrove plants.

AB - Mangrove species have high tolerance to heavy metal pollution. Chitinases have been widely reported as defense proteins in response to heavy metal stress in terrestrial plants. In this study, a full-length cDNA sequence encoding an acidic and basic class III chitinase (AmCHI III) was cloned by using RT-PCR and RACE methods in Avicennia marina. AmCHI III mRNA expression in leaf of A. marina were investigated under Cd, Pb stresses on using real-time quantitative PCR. The deduced AmCHI III protein consists of 302 amino acids, including a signal putative peptide region, and a catalytic domain. Homology modeling of the catalytic domain revealed a typical molecular structure of class III plant chitinases. Results further demonstrated that the regulation of AmCHI III mRNA expression in leaves was strongly dependent on Cd, Pb stresses. AmCHI III mRNA expressions were significantly increased in response to Cd, Pb, and peaked at 7 days Cd-exposure, 7 days Pb-exposure, respectively. AmCHI III mRNA expression exhibited more sensitive to Pb stress than Cd stress. This work was the first time cloing chitinase from A. marina, and it brought evidence on chitinase gene involving in heavy metals (Cd2+ and Pb2+) resistance or detoxification in plants. Further studies including the promoter and upstream regulation, gene over-expression and the response of mangrove chitinases to other stresses will shed more light on the role of chitinase in mangrove plants.

KW - Aricennia marina

KW - Cadmium

KW - Chitinase

KW - Clone

KW - Gene expression

KW - Lead

KW - Mangrove plants

UR - http://www.scopus.com/inward/record.url?scp=84948105748&partnerID=8YFLogxK

U2 - 10.1007/s10646-015-1501-1

DO - 10.1007/s10646-015-1501-1

M3 - 文章

C2 - 26044930

AN - SCOPUS:84948105748

SN - 0963-9292

VL - 24

SP - 1697

EP - 1704

JO - Ecotoxicology

JF - Ecotoxicology

IS - 7-8

ER -