Methanogenic biodegradation of C13 and C14 n-alkanes activated by addition to fumarate

Jia Heng Ji; Yi Fan Liu; Lei Zhou; Muhammad Irfan; Serge Maurice Mbadinga; Pan Pan; Jing Chen; Jin Feng Liu; Shi Zhong Yang; Wolfgang Sand; Ji Dong Gu; Bo Zhong Mu

doi:10.1016/j.ibiod.2020.104994

Methanogenic biodegradation of C₁₃ and C₁₄ n-alkanes activated by addition to fumarate

Jia Heng Ji, Yi Fan Liu, Lei Zhou, Muhammad Irfan, Serge Maurice Mbadinga, Pan Pan, Jing Chen, Jin Feng Liu, Shi Zhong Yang, Wolfgang Sand, Ji Dong Gu, Bo Zhong Mu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

n-Alkanes comprise a substantial proportion of crude oil; however, their degradation mechanism under methanogenic conditions is still unclear. Here, we investigated the initial activation mechanism of biodegradation of n-tridecane and n-tetradecane by methanogenic cultures enriched from a low-temperature oilfield production water. Methane accumulated in alkane-degrading enrichment cultures during the whole incubation period of 364 days, demonstrating that alkanes were actively degraded. Putative metabolites consistent with succinylated n-tridecane and n-tetradecane were detected, together with alkylsuccinate synthase-like genes, suggesting that alkanes were initially activated upon the addition of fumarate. Smithella and Desulfatibacillum in conjunction with hydrogenotrophic and acetoclastic methanogens were proposed to be important alkane degraders under methanogenic conditions. The results of this work added evidence that fumarate addition is a key activation mechanism in methanogenic alkane degradation.

Original language	English
Article number	104994
Journal	International Biodeterioration and Biodegradation
Volume	153
DOIs	https://doi.org/10.1016/j.ibiod.2020.104994
State	Published - Sep 2020
Externally published	Yes

Keywords

1-methylalkyl succinate
Alkane
Fumarate addition
Methanogenesis
Smithella

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@article{1d7cebf2646e4f899b46a8163b0191f6,

title = "Methanogenic biodegradation of C13 and C14 n-alkanes activated by addition to fumarate",

abstract = "n-Alkanes comprise a substantial proportion of crude oil; however, their degradation mechanism under methanogenic conditions is still unclear. Here, we investigated the initial activation mechanism of biodegradation of n-tridecane and n-tetradecane by methanogenic cultures enriched from a low-temperature oilfield production water. Methane accumulated in alkane-degrading enrichment cultures during the whole incubation period of 364 days, demonstrating that alkanes were actively degraded. Putative metabolites consistent with succinylated n-tridecane and n-tetradecane were detected, together with alkylsuccinate synthase-like genes, suggesting that alkanes were initially activated upon the addition of fumarate. Smithella and Desulfatibacillum in conjunction with hydrogenotrophic and acetoclastic methanogens were proposed to be important alkane degraders under methanogenic conditions. The results of this work added evidence that fumarate addition is a key activation mechanism in methanogenic alkane degradation.",

keywords = "1-methylalkyl succinate, Alkane, Fumarate addition, Methanogenesis, Smithella",

author = "Ji, {Jia Heng} and Liu, {Yi Fan} and Lei Zhou and Muhammad Irfan and Mbadinga, {Serge Maurice} and Pan Pan and Jing Chen and Liu, {Jin Feng} and Yang, {Shi Zhong} and Wolfgang Sand and Gu, {Ji Dong} and Mu, {Bo Zhong}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = sep,

doi = "10.1016/j.ibiod.2020.104994",

language = "英语",

volume = "153",

journal = "International Biodeterioration and Biodegradation",

issn = "0964-8305",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Methanogenic biodegradation of C13 and C14 n-alkanes activated by addition to fumarate

AU - Ji, Jia Heng

AU - Liu, Yi Fan

AU - Zhou, Lei

AU - Irfan, Muhammad

AU - Mbadinga, Serge Maurice

AU - Pan, Pan

AU - Chen, Jing

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Sand, Wolfgang

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

PY - 2020/9

Y1 - 2020/9

N2 - n-Alkanes comprise a substantial proportion of crude oil; however, their degradation mechanism under methanogenic conditions is still unclear. Here, we investigated the initial activation mechanism of biodegradation of n-tridecane and n-tetradecane by methanogenic cultures enriched from a low-temperature oilfield production water. Methane accumulated in alkane-degrading enrichment cultures during the whole incubation period of 364 days, demonstrating that alkanes were actively degraded. Putative metabolites consistent with succinylated n-tridecane and n-tetradecane were detected, together with alkylsuccinate synthase-like genes, suggesting that alkanes were initially activated upon the addition of fumarate. Smithella and Desulfatibacillum in conjunction with hydrogenotrophic and acetoclastic methanogens were proposed to be important alkane degraders under methanogenic conditions. The results of this work added evidence that fumarate addition is a key activation mechanism in methanogenic alkane degradation.

AB - n-Alkanes comprise a substantial proportion of crude oil; however, their degradation mechanism under methanogenic conditions is still unclear. Here, we investigated the initial activation mechanism of biodegradation of n-tridecane and n-tetradecane by methanogenic cultures enriched from a low-temperature oilfield production water. Methane accumulated in alkane-degrading enrichment cultures during the whole incubation period of 364 days, demonstrating that alkanes were actively degraded. Putative metabolites consistent with succinylated n-tridecane and n-tetradecane were detected, together with alkylsuccinate synthase-like genes, suggesting that alkanes were initially activated upon the addition of fumarate. Smithella and Desulfatibacillum in conjunction with hydrogenotrophic and acetoclastic methanogens were proposed to be important alkane degraders under methanogenic conditions. The results of this work added evidence that fumarate addition is a key activation mechanism in methanogenic alkane degradation.

KW - 1-methylalkyl succinate

KW - Alkane

KW - Fumarate addition

KW - Methanogenesis

KW - Smithella

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U2 - 10.1016/j.ibiod.2020.104994

DO - 10.1016/j.ibiod.2020.104994

M3 - 文章

AN - SCOPUS:85086072264

SN - 0964-8305

VL - 153

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 104994

ER -

Methanogenic biodegradation of C₁₃ and C₁₄ n-alkanes activated by addition to fumarate

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Keywords

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