Metabolism of diethyl phthalate (DEP) and identification of degradation intermediates by Pseudomonas sp. DNE-S1

TY - JOUR

T1 - Metabolism of diethyl phthalate (DEP) and identification of degradation intermediates by Pseudomonas sp. DNE-S1

AU - Tao, Yue

AU - Li, Hanxu

AU - Gu, Jidong

AU - Shi, Hongtao

AU - Han, Siyue

AU - Jiao, Yaqi

AU - Zhong, Guanyu

AU - Zhang, Qi

AU - Akindolie, Modupe S.

AU - Lin, Yulong

AU - Chen, Zhaobo

AU - Zhang, Ying

N1 - Publisher Copyright: © 2019 Elsevier Inc.

PY - 2019/5/30

Y1 - 2019/5/30

N2 - A Pseudomonas sp. DNE-S1 (GenBank accession number MF803832), able to degrade DEP in a wide range of acid-base conditions, was isolated from landfill soil. The growth kinetics of DNE-S1 on DEP followed the inhibition model. Fe 3+ could promote the degradation ability of DNE-S1 to DEP probably by over-expression of the gene phthalate dihydrodiol dehydrogenase (ophB) and phthalate dioxygenase ferredoxin reductase (ophA4). The degradation rate of DEP (500 mg L −1 at 12 h) increased by 14.5% in the presence of Fe 3+ . Cu 2+ , Zn 2+ , and Mn 2+ showed an inhibiting effect on the degradation performance of the strain and could alter the cellular morphology, surface area and volume of DNE-S1. Three degradation intermediates, namely ethyl methyl phthalate (EMP), dimethyl phthalate (DMP), and phthalic acid (PA), were detected in the biodegradation of DEP, and the biochemical pathway of DEP degradation was proposed. This study provides new information on the biochemical pathways and the responsible genes involved in DEP degradation.

AB - A Pseudomonas sp. DNE-S1 (GenBank accession number MF803832), able to degrade DEP in a wide range of acid-base conditions, was isolated from landfill soil. The growth kinetics of DNE-S1 on DEP followed the inhibition model. Fe 3+ could promote the degradation ability of DNE-S1 to DEP probably by over-expression of the gene phthalate dihydrodiol dehydrogenase (ophB) and phthalate dioxygenase ferredoxin reductase (ophA4). The degradation rate of DEP (500 mg L −1 at 12 h) increased by 14.5% in the presence of Fe 3+ . Cu 2+ , Zn 2+ , and Mn 2+ showed an inhibiting effect on the degradation performance of the strain and could alter the cellular morphology, surface area and volume of DNE-S1. Three degradation intermediates, namely ethyl methyl phthalate (EMP), dimethyl phthalate (DMP), and phthalic acid (PA), were detected in the biodegradation of DEP, and the biochemical pathway of DEP degradation was proposed. This study provides new information on the biochemical pathways and the responsible genes involved in DEP degradation.

KW - Biodegradation

KW - Cell morphology

KW - DEP

KW - Inhibition kinetics

KW - Microelements

UR - http://www.scopus.com/inward/record.url?scp=85061782351&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2019.02.055

DO - 10.1016/j.ecoenv.2019.02.055

M3 - 文章

C2 - 30798184

AN - SCOPUS:85061782351

SN - 0147-6513

VL - 173

SP - 411

EP - 418

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

ER -