Mangrove trees affect the community structure and distribution of anammox bacteria at an anthropogenic-polluted mangrove in the Pearl River Delta reflected by 16S rRNA and hydrazine oxidoreductase (HZO) encoding gene analyses

TY - JOUR

T1 - Mangrove trees affect the community structure and distribution of anammox bacteria at an anthropogenic-polluted mangrove in the Pearl River Delta reflected by 16S rRNA and hydrazine oxidoreductase (HZO) encoding gene analyses

AU - Li, Meng

AU - Hong, Yi Guo

AU - Cao, Hui Luo

AU - Gu, Ji Dong

N1 - Funding Information: Project of The Chinese Academy of Sciences (KZCX2-YW-QN207), National Natural Science Foundation of China (3080032), Guangdong Province Natural Science Foundation (84510301001692), and a start-up fund for Excellent Scholarship of the Chinese Academy of Sciences (07YQ091001) (to Y-G H). We would like to thank Jessie Lai for her laboratory assistance at The University of Hong Kong. Funding Information: Acknowledgments This research was supported in part by grants from Agriculture, Fisheries, and Conservation Department of the Hong Kong Government (to J-DG), Knowledge Innovation Key

PY - 2011/11

Y1 - 2011/11

N2 - Anaerobic ammonium oxidizing (anammox) bacterial community structures were investigated in surface (1-2 cm) and lower (20-21 cm) layers of mangrove sediments at sites located immediately to the mangrove trees (S0), 10 m (S1) and 1000 m (S2) away from mangrove trees in a polluted area of the Pearl River Delta. At S0, both 16S rRNA and hydrazine oxidoreductase (HZO) encoding genes of anammox bacteria showed high diversity in lower layer sediments, but they were not detectable in lower layer sediments in mangrove forest. S1 and S2 shared similar anammox bacteria communities in both surface and lower layers, which were quite different from that of S0. At all three locations, higher richness of anammox bacteria was detected in the surface layer than the lower layer; 16S rRNA genes revealed anammox bacteria were composed by four phylogenetic clusters affiliated with the "Scalindua" genus, and one group related to the potential anammox bacteria; while the hzo genes showed that in addition to sequences related to the "Scalindua", sequences affiliated with genera of "Kuenenia", "Brocadia", and "Jettenia" were also detected in mangrove sediments. Furthermore, hzo gene abundances decreased from 36.5 × 10 4 to 11.0 × 10 4 copies/gram dry sediment in lower layer sediments while increased from below detection limit to 31.5 × 10 4 copies/gram dry sediment in lower layer sediments from S0 to S2. The results indicated that anammox bacteria communities might be strongly influenced by mangrove trees. In addition, the correlation analysis showed the redox potential and the molar ratio of ammonium to nitrite in sediments might be important factors affecting the diversity and distribution of anammox bacteria in mangrove sediments.

AB - Anaerobic ammonium oxidizing (anammox) bacterial community structures were investigated in surface (1-2 cm) and lower (20-21 cm) layers of mangrove sediments at sites located immediately to the mangrove trees (S0), 10 m (S1) and 1000 m (S2) away from mangrove trees in a polluted area of the Pearl River Delta. At S0, both 16S rRNA and hydrazine oxidoreductase (HZO) encoding genes of anammox bacteria showed high diversity in lower layer sediments, but they were not detectable in lower layer sediments in mangrove forest. S1 and S2 shared similar anammox bacteria communities in both surface and lower layers, which were quite different from that of S0. At all three locations, higher richness of anammox bacteria was detected in the surface layer than the lower layer; 16S rRNA genes revealed anammox bacteria were composed by four phylogenetic clusters affiliated with the "Scalindua" genus, and one group related to the potential anammox bacteria; while the hzo genes showed that in addition to sequences related to the "Scalindua", sequences affiliated with genera of "Kuenenia", "Brocadia", and "Jettenia" were also detected in mangrove sediments. Furthermore, hzo gene abundances decreased from 36.5 × 10 4 to 11.0 × 10 4 copies/gram dry sediment in lower layer sediments while increased from below detection limit to 31.5 × 10 4 copies/gram dry sediment in lower layer sediments from S0 to S2. The results indicated that anammox bacteria communities might be strongly influenced by mangrove trees. In addition, the correlation analysis showed the redox potential and the molar ratio of ammonium to nitrite in sediments might be important factors affecting the diversity and distribution of anammox bacteria in mangrove sediments.

KW - 16S rRNA genes

KW - Abundances

KW - Anammox bacteria

KW - Distribution

KW - Diversity

KW - Mangrove

KW - hzo genes

UR - http://www.scopus.com/inward/record.url?scp=80054942788&partnerID=8YFLogxK

U2 - 10.1007/s10646-011-0711-4

DO - 10.1007/s10646-011-0711-4

M3 - 文章

C2 - 21735127

AN - SCOPUS:80054942788

SN - 0963-9292

VL - 20

SP - 1780

EP - 1790

JO - Ecotoxicology

JF - Ecotoxicology

IS - 8

ER -