L-tyrosine: An electrochemically-active Aqueous Humor (AH) antioxidant

S. P. Richer; B. Kramer; M. Eisen; R. C. Rose

L-tyrosine: An electrochemically-active Aqueous Humor (AH) antioxidant

S. P. Richer^*, B. Kramer, M. Eisen, R. C. Rose

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

PURPOSE : At ARVO 95 (#2422), we described HPLC/EC as a technique for simultaneously determining [H₂O₂], and the major water soluble antioxidants (WSAs) in NZ White rabbit AH: L-cysteine, L-ascorbic acid, glutathione and uric acid. Also reported, was an unknown species with a retention time (RT) of 13 min. METHODS and RESULTS: Based upon RT, I/V curve, pH titration, MW by dialysis and mass spec, chemical properties previously reported, and elimination with the enzyme mushroom tyrosinase, the unknown species is L-tyrosine, a known hydroxyl radical scavenger via aromatic hydroxylation. It's concentration at 100 uM is significant compared to other AH antioxidants, and second in electrochemical activity to AA. (Graph Presented). CONCLUSIONS: L-Tyrosine is an antioxidant at significant concentration in mammalian aqueous humor. Plans include determinination of "pecking order" for small MW aqueous humor WSAs in the physiol. relevant range of UVR and po₂. Support: EY0730, AOF and IL. Soc. Prev. Blindness.

Original language	English
Pages (from-to)	S580
Journal	Investigative Ophthalmology and Visual Science
Volume	37
Issue number	3
State	Published - 15 Feb 1996
Externally published	Yes

Cite this

@article{290c9f37ef004e83bb8944154524cba7,

title = "L-tyrosine: An electrochemically-active Aqueous Humor (AH) antioxidant",

abstract = "PURPOSE : At ARVO 95 (#2422), we described HPLC/EC as a technique for simultaneously determining [H2O2], and the major water soluble antioxidants (WSAs) in NZ White rabbit AH: L-cysteine, L-ascorbic acid, glutathione and uric acid. Also reported, was an unknown species with a retention time (RT) of 13 min. METHODS and RESULTS: Based upon RT, I/V curve, pH titration, MW by dialysis and mass spec, chemical properties previously reported, and elimination with the enzyme mushroom tyrosinase, the unknown species is L-tyrosine, a known hydroxyl radical scavenger via aromatic hydroxylation. It's concentration at 100 uM is significant compared to other AH antioxidants, and second in electrochemical activity to AA. (Graph Presented). CONCLUSIONS: L-Tyrosine is an antioxidant at significant concentration in mammalian aqueous humor. Plans include determinination of {"}pecking order{"} for small MW aqueous humor WSAs in the physiol. relevant range of UVR and po2. Support: EY0730, AOF and IL. Soc. Prev. Blindness.",

author = "Richer, {S. P.} and B. Kramer and M. Eisen and Rose, {R. C.}",

year = "1996",

month = feb,

day = "15",

language = "英语",

volume = "37",

pages = "S580",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "3",

}

TY - JOUR

T1 - L-tyrosine

T2 - An electrochemically-active Aqueous Humor (AH) antioxidant

AU - Richer, S. P.

AU - Kramer, B.

AU - Eisen, M.

AU - Rose, R. C.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - PURPOSE : At ARVO 95 (#2422), we described HPLC/EC as a technique for simultaneously determining [H2O2], and the major water soluble antioxidants (WSAs) in NZ White rabbit AH: L-cysteine, L-ascorbic acid, glutathione and uric acid. Also reported, was an unknown species with a retention time (RT) of 13 min. METHODS and RESULTS: Based upon RT, I/V curve, pH titration, MW by dialysis and mass spec, chemical properties previously reported, and elimination with the enzyme mushroom tyrosinase, the unknown species is L-tyrosine, a known hydroxyl radical scavenger via aromatic hydroxylation. It's concentration at 100 uM is significant compared to other AH antioxidants, and second in electrochemical activity to AA. (Graph Presented). CONCLUSIONS: L-Tyrosine is an antioxidant at significant concentration in mammalian aqueous humor. Plans include determinination of "pecking order" for small MW aqueous humor WSAs in the physiol. relevant range of UVR and po2. Support: EY0730, AOF and IL. Soc. Prev. Blindness.

AB - PURPOSE : At ARVO 95 (#2422), we described HPLC/EC as a technique for simultaneously determining [H2O2], and the major water soluble antioxidants (WSAs) in NZ White rabbit AH: L-cysteine, L-ascorbic acid, glutathione and uric acid. Also reported, was an unknown species with a retention time (RT) of 13 min. METHODS and RESULTS: Based upon RT, I/V curve, pH titration, MW by dialysis and mass spec, chemical properties previously reported, and elimination with the enzyme mushroom tyrosinase, the unknown species is L-tyrosine, a known hydroxyl radical scavenger via aromatic hydroxylation. It's concentration at 100 uM is significant compared to other AH antioxidants, and second in electrochemical activity to AA. (Graph Presented). CONCLUSIONS: L-Tyrosine is an antioxidant at significant concentration in mammalian aqueous humor. Plans include determinination of "pecking order" for small MW aqueous humor WSAs in the physiol. relevant range of UVR and po2. Support: EY0730, AOF and IL. Soc. Prev. Blindness.

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M3 - 文章

AN - SCOPUS:2542435117

SN - 0146-0404

VL - 37

SP - S580

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 3

ER -

L-tyrosine: An electrochemically-active Aqueous Humor (AH) antioxidant

Abstract

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