TY - JOUR
T1 - Kinetics of n-butyl benzyl phthalate degradation by a pure bacterial culture from the mangrove sediment
AU - Xu, Xiang Rong
AU - Li, Hua Bin
AU - Gu, Ji Dong
AU - Li, Xiao Yan
PY - 2007/2/9
Y1 - 2007/2/9
N2 - n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP by a bacterium, Pseudomonas fluorescens B-1, isolated from the mangrove sediment, was investigated. The microorganism can utilize BBP as the sole carbon and energy source, where concentrations of BBP disappeared within 6 days under shake culture conditions. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied, respectively. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid and benzoic acid by gas chromatography-mass spectrometry, and a preliminary metabolic pathway of BBP was proposed.
AB - n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP by a bacterium, Pseudomonas fluorescens B-1, isolated from the mangrove sediment, was investigated. The microorganism can utilize BBP as the sole carbon and energy source, where concentrations of BBP disappeared within 6 days under shake culture conditions. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied, respectively. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid and benzoic acid by gas chromatography-mass spectrometry, and a preliminary metabolic pathway of BBP was proposed.
KW - Biodegradation
KW - Endocrine-disruptor
KW - Kinetics
KW - Pseudomonas fluorescens B-1
KW - n-Butyl benzyl phthalate
UR - http://www.scopus.com/inward/record.url?scp=33846994747&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2006.06.054
DO - 10.1016/j.jhazmat.2006.06.054
M3 - 文章
C2 - 16876944
AN - SCOPUS:33846994747
SN - 0304-3894
VL - 140
SP - 194
EP - 199
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
IS - 1-2
ER -