Kinetics of di-n-butyl phthalate degradation by a bacterium isolated from mangrove sediment

Xiang Rong Xu, Ji Dong Gu*, Hua Bin Li, Xiao Yan Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Biodegradation of the endocrine-disrupting chemical di-n-butyl phthalate (DBP) was investigated using a bacterium, Pseudomonas fluorescens B-1, isolated from mangrove sediment. The effects of temperature, pH, salinity, and oxygen availability on DBP degradation were studied. Degradation of DBP was monitored by solid-phase extraction using reversed-phase HPLC and UV detection. The major metabolites of DBP degradation were identified as mono-n-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry (GC-MS) and a pathway of degradation was proposed. Degradation by P. fluorescens B-1 conformed to first-order kinetics. Degradation of DBP was also tested in seawater by inoculating P. fluorescens B-1, and complete degradation of an initial concentration of 100 μg/l was achieved in 144 h. These results suggest that DBP is readily degraded by bacteria in natural environments.

Original languageEnglish
Pages (from-to)946-951
Number of pages6
JournalJournal of Microbiology and Biotechnology
Issue number5
StatePublished - Oct 2005
Externally publishedYes


  • Biodegradation
  • Di-n-butyl phthalate
  • Kinetics
  • Mechanism
  • Phthalates


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