TY - JOUR
T1 - Insights into the bacterial and fungal communities and microbiome that causes a microbe outbreak on ancient wall paintings in the Maijishan Grottoes
AU - He, Dongpeng
AU - Wu, Fasi
AU - Ma, Wenxia
AU - Zhang, Yong
AU - Gu, Ji Dong
AU - Duan, Yulong
AU - Xu, Ruihong
AU - Feng, Huyuan
AU - Wang, Wanfu
AU - Li, Shi Weng
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/9
Y1 - 2021/9
N2 - The Maijishan Grottoes, located on the ancient Silk Road in West China, was constructed from the 4th centuries onward for more than 1500 years and became a UNESCO World Cultural Heritage in 2014. A severe microbe outbreak occurred on the wall paintings in the caves of the Maijishan Grottoes in 2018. To prevent and control microbial damage to the wall paintings, we analyzed the bacterial and fungal communities in the normal wall samples and those with microbial plaques using high-throughput sequencing and the environmental factors associated with the microbe outbreak. The results showed that the normal wall samples were dominated by bacterial phyla Actinobacteria (71.7%), Proteobacteria (17.8%), Firmicutes (6.5%), Chloroflexi (2.4%), and Bacteroidetes (0.9%), and fungal families Trichocomaceae (43.4%), unclassified Capnodiales (23.2%), unclassified Ascomycota (12.1%), Teratosphaeriaceae (10.2%), and unclassified Eurotiomycetes (6.2%). The wall samples with microbial plaques were dominated by bacterial Actinobacteria (92.9%), Proteobacteria (5.3%), Firmicutes (1.1%), and Chloroflexi (0.3%) and fungal families unclassified Eurotiomycetes (93.3%), unclassified Ascomycota (3.3%), and Microascaceae (2.9%). These results indicated the great changes in bacterial and fungal communities during the microbe outbreak. The dominant fungus in the wall samples with microbial plaques was isolated and identified as a potential novel species which has 92% ITS sequence similarity with Arachnomyces sp. Canonical correspondence analysis (CCA) revealed that the microbe outbreak was primarily attributed to the excessive air humidity inside and outside of the caves. Thus, air humidity management is important for protecting the ancient wall paintings from microbial colonization and attack.
AB - The Maijishan Grottoes, located on the ancient Silk Road in West China, was constructed from the 4th centuries onward for more than 1500 years and became a UNESCO World Cultural Heritage in 2014. A severe microbe outbreak occurred on the wall paintings in the caves of the Maijishan Grottoes in 2018. To prevent and control microbial damage to the wall paintings, we analyzed the bacterial and fungal communities in the normal wall samples and those with microbial plaques using high-throughput sequencing and the environmental factors associated with the microbe outbreak. The results showed that the normal wall samples were dominated by bacterial phyla Actinobacteria (71.7%), Proteobacteria (17.8%), Firmicutes (6.5%), Chloroflexi (2.4%), and Bacteroidetes (0.9%), and fungal families Trichocomaceae (43.4%), unclassified Capnodiales (23.2%), unclassified Ascomycota (12.1%), Teratosphaeriaceae (10.2%), and unclassified Eurotiomycetes (6.2%). The wall samples with microbial plaques were dominated by bacterial Actinobacteria (92.9%), Proteobacteria (5.3%), Firmicutes (1.1%), and Chloroflexi (0.3%) and fungal families unclassified Eurotiomycetes (93.3%), unclassified Ascomycota (3.3%), and Microascaceae (2.9%). These results indicated the great changes in bacterial and fungal communities during the microbe outbreak. The dominant fungus in the wall samples with microbial plaques was isolated and identified as a potential novel species which has 92% ITS sequence similarity with Arachnomyces sp. Canonical correspondence analysis (CCA) revealed that the microbe outbreak was primarily attributed to the excessive air humidity inside and outside of the caves. Thus, air humidity management is important for protecting the ancient wall paintings from microbial colonization and attack.
KW - Bacterial community
KW - Fungal community
KW - Grottoes
KW - Microbe outbreak
KW - Microbial colonization
UR - http://www.scopus.com/inward/record.url?scp=85108990671&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2021.105250
DO - 10.1016/j.ibiod.2021.105250
M3 - 文章
AN - SCOPUS:85108990671
SN - 0964-8305
VL - 163
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
M1 - 105250
ER -