Inhibition of cholesterol transport into skin cells in cultures by phytosterol-loaded microemulsion

TY - JOUR

T1 - Inhibition of cholesterol transport into skin cells in cultures by phytosterol-loaded microemulsion

AU - Rozner, S.

AU - Verkhovski, L.

AU - Nissimov, Y.

AU - Aserin, A.

AU - Vilensky, R.

AU - Danino, D.

AU - Zouboulis, C. C.

AU - Milner, Y.

AU - Garti, N.

N1 - Funding Information: This work was partially supported by the Inez and David Myers Foundation of Cleveland, Ohio (to YM). DD acknowledges the financial support of the ISF, and the RBNI. NG and DD acknowledge BSF (US-Israel Binational Science Foundation) for their financial support.

PY - 2008/6

Y1 - 2008/6

N2 - Cholesterol and plant phytosterols are lipophilic compounds solubilized by intestinal micelles in a competitive manner. In this work, we used radioactive cholesterol- and phytosterol-loaded oil-in-water microemulsions to follow their incorporation and mutual competition in HaCaT keratinocytes, SZ95 sebocytes, and skin pieces in cultures. Dynamic light scattering showed homogenous nanostructures of 10.5 ± 1.5 nm diameter and cryo-transmission electron microscopy confirmed the presence of uniform spherical droplets of 7.0 ± 1.0 nm diameter. Up to 320 nmol/ml of cholesterol can be solubilized and transported into cells with minimal toxic effect by 0.5 wt% nanodroplets in a cell medium. Phytosterols inhibit incorporation of cholesterol into cells, in vitro, at molar ratios (phytosterols/cholesterol) of 4 and above. The loaded nanodroplets accumulate in intracellular vesicles (presumably endosomes). No metabolic conversion of cholesterol or phytosterols was found in these cells, in vitro, after 24 h, at 37 °C.

AB - Cholesterol and plant phytosterols are lipophilic compounds solubilized by intestinal micelles in a competitive manner. In this work, we used radioactive cholesterol- and phytosterol-loaded oil-in-water microemulsions to follow their incorporation and mutual competition in HaCaT keratinocytes, SZ95 sebocytes, and skin pieces in cultures. Dynamic light scattering showed homogenous nanostructures of 10.5 ± 1.5 nm diameter and cryo-transmission electron microscopy confirmed the presence of uniform spherical droplets of 7.0 ± 1.0 nm diameter. Up to 320 nmol/ml of cholesterol can be solubilized and transported into cells with minimal toxic effect by 0.5 wt% nanodroplets in a cell medium. Phytosterols inhibit incorporation of cholesterol into cells, in vitro, at molar ratios (phytosterols/cholesterol) of 4 and above. The loaded nanodroplets accumulate in intracellular vesicles (presumably endosomes). No metabolic conversion of cholesterol or phytosterols was found in these cells, in vitro, after 24 h, at 37 °C.

KW - Incorporation

KW - Keratinocyte cells

KW - Metabolism

KW - Microemulsion

KW - Phytosterols

KW - Skin cultures

UR - http://www.scopus.com/inward/record.url?scp=43049178202&partnerID=8YFLogxK

U2 - 10.1016/j.chemphyslip.2008.02.017

DO - 10.1016/j.chemphyslip.2008.02.017

M3 - 文章

C2 - 18395012

AN - SCOPUS:43049178202

SN - 0009-3084

VL - 153

SP - 109

EP - 118

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

IS - 2

ER -