Improved enzyme production by co-cultivation of Aspergillus niger and Aspergillus oryzae and with other fungi

TY - JOUR

T1 - Improved enzyme production by co-cultivation of Aspergillus niger and Aspergillus oryzae and with other fungi

AU - Hu, H. L.

AU - van den Brink, J.

AU - Gruben, B. S.

AU - Wösten, H. A.B.

AU - Gu, J. D.

AU - de Vries, R. P.

N1 - Funding Information: HHL thanks Utrecht University for providing her with a Short Stay Fellowship and The University of Hong Kong for the Postgraduate Studentship. BG and RPdV were supported by grants of the Dutch Foundation for Applied Science (STW) 07938 and 07063, respectively. The authors thank Sacha Dalhuijsen, Evy Battaglia and Denise de Jongh for technical assistance.

PY - 2011/1

Y1 - 2011/1

N2 - Aspergillus niger and Aspergillus oryzae were co-cultivated with each other and with Magnaporthe grisea or Phanerochaete chrysosporium, respectively. Enzyme assays for plant polysaccharide and lignin-degrading enzymes showed that co-cultivation can improve extracellular enzyme production. Highest β-glucosidase, α-cellobiohydrolase, β-galactosidase, and laccase activities were found for A. oryzae in combination with other fungi, in particular with P. chrysosporium. Highest β-xylosidase activity was obtained when A. niger was co-cultivated with P. chrysosporium. SDS-PAGE protein profiles demonstrated that A. niger and A. oryzae contributed most to the overall enzyme activities found in the culture medium of the mixed cultivations. These data demonstrate that co-cultivation of two major industrial fungi, A. niger and A. oryzae, results in improved production of biotechnologically relevant enzymes.

AB - Aspergillus niger and Aspergillus oryzae were co-cultivated with each other and with Magnaporthe grisea or Phanerochaete chrysosporium, respectively. Enzyme assays for plant polysaccharide and lignin-degrading enzymes showed that co-cultivation can improve extracellular enzyme production. Highest β-glucosidase, α-cellobiohydrolase, β-galactosidase, and laccase activities were found for A. oryzae in combination with other fungi, in particular with P. chrysosporium. Highest β-xylosidase activity was obtained when A. niger was co-cultivated with P. chrysosporium. SDS-PAGE protein profiles demonstrated that A. niger and A. oryzae contributed most to the overall enzyme activities found in the culture medium of the mixed cultivations. These data demonstrate that co-cultivation of two major industrial fungi, A. niger and A. oryzae, results in improved production of biotechnologically relevant enzymes.

KW - Aspergillus

KW - Co-cultivation

KW - Enzyme production

KW - Lignin degradation

KW - Polysaccharide degradation

UR - http://www.scopus.com/inward/record.url?scp=78650677398&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2010.11.008

DO - 10.1016/j.ibiod.2010.11.008

M3 - 文章

AN - SCOPUS:78650677398

SN - 0964-8305

VL - 65

SP - 248

EP - 252

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

IS - 1

ER -