TY - JOUR
T1 - HPLC characterization of betalains from plants in the Amaranthaceae
AU - Cai, Yizhong
AU - Sun, Mei
AU - Corke, Harold
N1 - Funding Information:
Genotypes of 37 species from eight genera (mainly Amaranthus, Celosia, Gomphrena, and Iresine) in the Amaranthaceae from world collections, mainly provided by the United States Department of Agriculture–Agricultural Research Service (Iowa State University, Iowa City, Iowa), Chinese Academy of Agricultural Sciences (Beijing, China), and Hubei Academy of Agricultural Sciences (Wuhan, China), were used as materials for this study. All samples of fresh materials were harvested, frozen, and stored at –18°C until use.
PY - 2005/10
Y1 - 2005/10
N2 - HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37 species of eight genera in the Amaranthaceae. A total of 16 betacyanins and three betaxanthins are characterized mainly using the RP-HPLC method and also with the aid of mass spectroscopy. The identified betacyanins include eight amaranthine-types, six gomphrenin-types, and two betanin-types. They are also divided into six simple (nonacylated) betacyanins and 10 acylated betacyanins. Acylated betacyanins are identified as betanidin 5-O-β-glucuronosylglucoside or betanidin 6-O-β-glucoside acylated with ferulic, p-coumaric, or 3-hydroxy-3-methylglutaric acids. Three betaxanthins were separated from Celosia species in the Amaranthaceae and identified to be immonium conjugates of betalamic acid with dopamine, 3-methoxytyramine, and (S)-tryptophan; the latter two are found to be new betaxanthins from plants.
AB - HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37 species of eight genera in the Amaranthaceae. A total of 16 betacyanins and three betaxanthins are characterized mainly using the RP-HPLC method and also with the aid of mass spectroscopy. The identified betacyanins include eight amaranthine-types, six gomphrenin-types, and two betanin-types. They are also divided into six simple (nonacylated) betacyanins and 10 acylated betacyanins. Acylated betacyanins are identified as betanidin 5-O-β-glucuronosylglucoside or betanidin 6-O-β-glucoside acylated with ferulic, p-coumaric, or 3-hydroxy-3-methylglutaric acids. Three betaxanthins were separated from Celosia species in the Amaranthaceae and identified to be immonium conjugates of betalamic acid with dopamine, 3-methoxytyramine, and (S)-tryptophan; the latter two are found to be new betaxanthins from plants.
UR - http://www.scopus.com/inward/record.url?scp=27744564210&partnerID=8YFLogxK
U2 - 10.1093/chromsci/43.9.454
DO - 10.1093/chromsci/43.9.454
M3 - 文章
C2 - 16212790
AN - SCOPUS:27744564210
SN - 0021-9665
VL - 43
SP - 454
EP - 460
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 9
ER -