Genetic Circuit-Assisted Smart Microbial Engineering

Cong Gao; Peng Xu; Chao Ye; Xiulai Chen; Liming Liu

doi:10.1016/j.tim.2019.07.005

Genetic Circuit-Assisted Smart Microbial Engineering

Cong Gao, Peng Xu, Chao Ye, Xiulai Chen, Liming Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

47 Scopus citations

Abstract

Rapid advances in DNA synthesis, genetic manipulation, and biosensors have greatly improved the ability to engineer microorganisms with complex functions. By accurately integrating quality biosensors and complex genetic circuits, recently emerged smart microorganisms have enabled exciting opportunities for dissecting complex signaling networks and making responses without artificial intervention. However, because of the lack of design principles, developing such smart microorganisms remains challenging. In this review, we propose the concept of smart microbial engineering (SME) and describe the general features of basic SME, including the circuit architecture, components, and design process. We also summarize the latest SME achievements, remaining challenges, and potential solutions in this growing field.

Original language	English
Pages (from-to)	1011-1024
Number of pages	14
Journal	Trends in Microbiology
Volume	27
Issue number	12
DOIs	https://doi.org/10.1016/j.tim.2019.07.005
State	Published - Dec 2019
Externally published	Yes

Keywords

applications
genetic circuits
metabolic engineering
microbial engineering
synthetic biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tim.2019.07.005

Cite this

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title = "Genetic Circuit-Assisted Smart Microbial Engineering",

abstract = "Rapid advances in DNA synthesis, genetic manipulation, and biosensors have greatly improved the ability to engineer microorganisms with complex functions. By accurately integrating quality biosensors and complex genetic circuits, recently emerged smart microorganisms have enabled exciting opportunities for dissecting complex signaling networks and making responses without artificial intervention. However, because of the lack of design principles, developing such smart microorganisms remains challenging. In this review, we propose the concept of smart microbial engineering (SME) and describe the general features of basic SME, including the circuit architecture, components, and design process. We also summarize the latest SME achievements, remaining challenges, and potential solutions in this growing field.",

keywords = "applications, genetic circuits, metabolic engineering, microbial engineering, synthetic biology",

author = "Cong Gao and Peng Xu and Chao Ye and Xiulai Chen and Liming Liu",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = dec,

doi = "10.1016/j.tim.2019.07.005",

language = "英语",

volume = "27",

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T1 - Genetic Circuit-Assisted Smart Microbial Engineering

AU - Gao, Cong

AU - Xu, Peng

AU - Ye, Chao

AU - Chen, Xiulai

AU - Liu, Liming

PY - 2019/12

Y1 - 2019/12

N2 - Rapid advances in DNA synthesis, genetic manipulation, and biosensors have greatly improved the ability to engineer microorganisms with complex functions. By accurately integrating quality biosensors and complex genetic circuits, recently emerged smart microorganisms have enabled exciting opportunities for dissecting complex signaling networks and making responses without artificial intervention. However, because of the lack of design principles, developing such smart microorganisms remains challenging. In this review, we propose the concept of smart microbial engineering (SME) and describe the general features of basic SME, including the circuit architecture, components, and design process. We also summarize the latest SME achievements, remaining challenges, and potential solutions in this growing field.

AB - Rapid advances in DNA synthesis, genetic manipulation, and biosensors have greatly improved the ability to engineer microorganisms with complex functions. By accurately integrating quality biosensors and complex genetic circuits, recently emerged smart microorganisms have enabled exciting opportunities for dissecting complex signaling networks and making responses without artificial intervention. However, because of the lack of design principles, developing such smart microorganisms remains challenging. In this review, we propose the concept of smart microbial engineering (SME) and describe the general features of basic SME, including the circuit architecture, components, and design process. We also summarize the latest SME achievements, remaining challenges, and potential solutions in this growing field.

KW - applications

KW - genetic circuits

KW - metabolic engineering

KW - microbial engineering

KW - synthetic biology

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U2 - 10.1016/j.tim.2019.07.005

DO - 10.1016/j.tim.2019.07.005

M3 - 文献综述

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AN - SCOPUS:85070538188

SN - 0966-842X

VL - 27

SP - 1011

EP - 1024

JO - Trends in Microbiology

JF - Trends in Microbiology

IS - 12

ER -

Genetic Circuit-Assisted Smart Microbial Engineering

Abstract

Keywords

UN SDGs

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