Gene abundances of AOA, AOB, and anammox controlled by groundwater chemistry of the Pearl River Delta, China

TY - JOUR

T1 - Gene abundances of AOA, AOB, and anammox controlled by groundwater chemistry of the Pearl River Delta, China

AU - Liu, Kun

AU - Luo, Xin

AU - Jiao, Jiu Jimmy

AU - Gu, Ji dong

AU - Aravena, Ramon

N1 - Publisher Copyright: © 2021 Editorial Office of China Geology. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

PY - 2021/9

Y1 - 2021/9

N2 - Ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and anaerobic ammoniaoxidation (anammox) bacteria are very important contributors to nitrogen cycling in natural environments. Functional gene abundances of these microbes were believed to be well relevant to N-cycling in groundwater systems, especially in the Pearl River Delta (PRD) groundwater with unique high intrinsic ammonia concentrations. In this research, 20 sediment samples from two in the PRD were collected for porewater chemistry analysis and quantification of N-cycling related genes, including archaeal and bacterial amoA gene and anammox 16S ribosomal Ribonucleic Acid (rRNA) gene. Quantitative Polymerase Chain Reaction (qPCR) results showed that gene abundances of AOA, AOB, and anammox bacteria ranged from 3.13×105 to 3.21×107, 1.83×104 to 2.74×106, and 9.27×104 to 8.96×106 copies/g in the sediment of the groundwater system, respectively. Anammox bacteria and AOA dominated in aquitards and aquifers, respectively, meanwhile, the aquitard-aquifer interfaces were demonstrated as ammonium-oxidizing hotspots in the aspect of gene numbers. Gene abundances of nitrifiers were analyzed with geochemistry profiles. Correlations between gene numbers and environmental variables indicated that the gene abundances were impacted by hydrogeological conditions, and microbial-derived ammonium loss was dominated by AOA in the northwest PRD and by anammox bacteria in the southeast PRD.

AB - Ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and anaerobic ammoniaoxidation (anammox) bacteria are very important contributors to nitrogen cycling in natural environments. Functional gene abundances of these microbes were believed to be well relevant to N-cycling in groundwater systems, especially in the Pearl River Delta (PRD) groundwater with unique high intrinsic ammonia concentrations. In this research, 20 sediment samples from two in the PRD were collected for porewater chemistry analysis and quantification of N-cycling related genes, including archaeal and bacterial amoA gene and anammox 16S ribosomal Ribonucleic Acid (rRNA) gene. Quantitative Polymerase Chain Reaction (qPCR) results showed that gene abundances of AOA, AOB, and anammox bacteria ranged from 3.13×105 to 3.21×107, 1.83×104 to 2.74×106, and 9.27×104 to 8.96×106 copies/g in the sediment of the groundwater system, respectively. Anammox bacteria and AOA dominated in aquitards and aquifers, respectively, meanwhile, the aquitard-aquifer interfaces were demonstrated as ammonium-oxidizing hotspots in the aspect of gene numbers. Gene abundances of nitrifiers were analyzed with geochemistry profiles. Correlations between gene numbers and environmental variables indicated that the gene abundances were impacted by hydrogeological conditions, and microbial-derived ammonium loss was dominated by AOA in the northwest PRD and by anammox bacteria in the southeast PRD.

KW - Ammonium oxidation bacteria (AOB)

KW - Ammonium oxidizing archaea (AOA)

KW - Anammox

KW - Aquitard

KW - China

KW - Groundwater

KW - Hydrogeological survey engineering

KW - Pearl River Delta

UR - http://www.scopus.com/inward/record.url?scp=85119453473&partnerID=8YFLogxK

U2 - 10.31035/cg2021054

DO - 10.31035/cg2021054

M3 - 文章

AN - SCOPUS:85119453473

SN - 2096-5192

VL - 4

SP - 463

EP - 475

JO - China Geology

JF - China Geology

IS - 3

ER -