Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir

Lei Zhou; Da Wei Wang; Shi Lun Zhang; En Gao Tang; Yu Wei Lu; Ya Fei Jing; Dan Dan Lin; Zhong Lin Liu; Jin Feng Liu; Shi Zhong Yang; Jian Zhang; Ji Dong Gu; Bo Zhong Mu

doi:10.1016/j.ibiod.2020.105057

Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir

Lei Zhou, Da Wei Wang, Shi Lun Zhang, En Gao Tang, Yu Wei Lu, Ya Fei Jing, Dan Dan Lin, Zhong Lin Liu, Jin Feng Liu, Shi Zhong Yang, Jian Zhang^*, Ji Dong Gu, Bo Zhong Mu^*

^*Corresponding author for this work

Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Microorganisms with diverse metabolic functions inhabit petroleum reservoir systems utilizing multiple electron acceptors, but current understanding about the composition and diversity of functional microbial community in offshore oilfields is still scarce. In this study, the diversity, phylogenetic distribution and abundance of microbes involved in the sulfur (S) and nitrogen (N) metabolisms in production water of a high-temperature offshore oilfield were described selectively based on the functional gene analysis using polymerase chain reaction (PCR) technique. The detected sulfate-reducing prokaryotes (SRP) were affiliated with δ-Proteobacteria, Thermodesulfobacteria, Nitrospira, Firmicutes and Euryarchaeota, and the key and dominant SRP in the two samples included the genera Archaeoglobus, Thermodesulfobacterium, Thermodesulfovibrio, Thermodesulforhabdus and Desulfomicrobium. The sulfur-oxidizing prokaryotes (SOP) belonged to α-, β- and γ-Proteobacteria, mainly represented by Roseovarius, Rhizobium, Methylobacterium, Thiobacillus and Thauera genera. In addition, the nitrate-reducing and denitrifying communities were composed of α-, β- and γ-Proteobacteria, among which Bosea, Pseudomonas, Marinobacter, Diaphorobacter and Mesorhizobium showed a relatively high abundance. The quantitative PCR results revealed that functional gene abundances of N metabolism (the napA, nirS and nosZ genes) were mostly higher than those of S metabolism (the aprA, dsrA, dsrB and soxB genes), and functional gene abundances in sample X1 were mostly higher than those in sample X2. This work provides basic data for the understanding of diversity, composition and distribution of functional microorganisms in offshore petroleum reservoir ecosystems, and also mitigation of the reservoir souring and control.

Original language	English
Article number	105057
Journal	International Biodeterioration and Biodegradation
Volume	154
DOIs	https://doi.org/10.1016/j.ibiod.2020.105057
State	Published - Oct 2020

Keywords

Denitrifier
Functional gene
Nitrate-reducing bacteria
Offshore petroleum reservoir
Sulfate-reducing prokaryote
Sulfur-oxidizing prokaryote

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Zhou, L., Wang, D. W., Zhang, S. L., Tang, E. G., Lu, Y. W., Jing, Y. F., Lin, D. D., Liu, Z. L., Liu, J. F., Yang, S. Z., Zhang, J., Gu, J. D., & Mu, B. Z. (2020). Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir. International Biodeterioration and Biodegradation, 154, Article 105057. https://doi.org/10.1016/j.ibiod.2020.105057

@article{adbf416c75914ef1ac71835ff09b7fc9,

title = "Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir",

abstract = "Microorganisms with diverse metabolic functions inhabit petroleum reservoir systems utilizing multiple electron acceptors, but current understanding about the composition and diversity of functional microbial community in offshore oilfields is still scarce. In this study, the diversity, phylogenetic distribution and abundance of microbes involved in the sulfur (S) and nitrogen (N) metabolisms in production water of a high-temperature offshore oilfield were described selectively based on the functional gene analysis using polymerase chain reaction (PCR) technique. The detected sulfate-reducing prokaryotes (SRP) were affiliated with δ-Proteobacteria, Thermodesulfobacteria, Nitrospira, Firmicutes and Euryarchaeota, and the key and dominant SRP in the two samples included the genera Archaeoglobus, Thermodesulfobacterium, Thermodesulfovibrio, Thermodesulforhabdus and Desulfomicrobium. The sulfur-oxidizing prokaryotes (SOP) belonged to α-, β- and γ-Proteobacteria, mainly represented by Roseovarius, Rhizobium, Methylobacterium, Thiobacillus and Thauera genera. In addition, the nitrate-reducing and denitrifying communities were composed of α-, β- and γ-Proteobacteria, among which Bosea, Pseudomonas, Marinobacter, Diaphorobacter and Mesorhizobium showed a relatively high abundance. The quantitative PCR results revealed that functional gene abundances of N metabolism (the napA, nirS and nosZ genes) were mostly higher than those of S metabolism (the aprA, dsrA, dsrB and soxB genes), and functional gene abundances in sample X1 were mostly higher than those in sample X2. This work provides basic data for the understanding of diversity, composition and distribution of functional microorganisms in offshore petroleum reservoir ecosystems, and also mitigation of the reservoir souring and control.",

keywords = "Denitrifier, Functional gene, Nitrate-reducing bacteria, Offshore petroleum reservoir, Sulfate-reducing prokaryote, Sulfur-oxidizing prokaryote",

author = "Lei Zhou and Wang, {Da Wei} and Zhang, {Shi Lun} and Tang, {En Gao} and Lu, {Yu Wei} and Jing, {Ya Fei} and Lin, {Dan Dan} and Liu, {Zhong Lin} and Liu, {Jin Feng} and Yang, {Shi Zhong} and Jian Zhang and Gu, {Ji Dong} and Mu, {Bo Zhong}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = oct,

doi = "10.1016/j.ibiod.2020.105057",

language = "英语",

volume = "154",

journal = "International Biodeterioration and Biodegradation",

issn = "0964-8305",

publisher = "Elsevier Ltd.",

}

Zhou, L, Wang, DW, Zhang, SL, Tang, EG, Lu, YW, Jing, YF, Lin, DD, Liu, ZL, Liu, JF, Yang, SZ, Zhang, J, Gu, JD & Mu, BZ 2020, 'Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir', International Biodeterioration and Biodegradation, vol. 154, 105057. https://doi.org/10.1016/j.ibiod.2020.105057

TY - JOUR

T1 - Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir

AU - Zhou, Lei

AU - Wang, Da Wei

AU - Zhang, Shi Lun

AU - Tang, En Gao

AU - Lu, Yu Wei

AU - Jing, Ya Fei

AU - Lin, Dan Dan

AU - Liu, Zhong Lin

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Zhang, Jian

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

PY - 2020/10

Y1 - 2020/10

N2 - Microorganisms with diverse metabolic functions inhabit petroleum reservoir systems utilizing multiple electron acceptors, but current understanding about the composition and diversity of functional microbial community in offshore oilfields is still scarce. In this study, the diversity, phylogenetic distribution and abundance of microbes involved in the sulfur (S) and nitrogen (N) metabolisms in production water of a high-temperature offshore oilfield were described selectively based on the functional gene analysis using polymerase chain reaction (PCR) technique. The detected sulfate-reducing prokaryotes (SRP) were affiliated with δ-Proteobacteria, Thermodesulfobacteria, Nitrospira, Firmicutes and Euryarchaeota, and the key and dominant SRP in the two samples included the genera Archaeoglobus, Thermodesulfobacterium, Thermodesulfovibrio, Thermodesulforhabdus and Desulfomicrobium. The sulfur-oxidizing prokaryotes (SOP) belonged to α-, β- and γ-Proteobacteria, mainly represented by Roseovarius, Rhizobium, Methylobacterium, Thiobacillus and Thauera genera. In addition, the nitrate-reducing and denitrifying communities were composed of α-, β- and γ-Proteobacteria, among which Bosea, Pseudomonas, Marinobacter, Diaphorobacter and Mesorhizobium showed a relatively high abundance. The quantitative PCR results revealed that functional gene abundances of N metabolism (the napA, nirS and nosZ genes) were mostly higher than those of S metabolism (the aprA, dsrA, dsrB and soxB genes), and functional gene abundances in sample X1 were mostly higher than those in sample X2. This work provides basic data for the understanding of diversity, composition and distribution of functional microorganisms in offshore petroleum reservoir ecosystems, and also mitigation of the reservoir souring and control.

AB - Microorganisms with diverse metabolic functions inhabit petroleum reservoir systems utilizing multiple electron acceptors, but current understanding about the composition and diversity of functional microbial community in offshore oilfields is still scarce. In this study, the diversity, phylogenetic distribution and abundance of microbes involved in the sulfur (S) and nitrogen (N) metabolisms in production water of a high-temperature offshore oilfield were described selectively based on the functional gene analysis using polymerase chain reaction (PCR) technique. The detected sulfate-reducing prokaryotes (SRP) were affiliated with δ-Proteobacteria, Thermodesulfobacteria, Nitrospira, Firmicutes and Euryarchaeota, and the key and dominant SRP in the two samples included the genera Archaeoglobus, Thermodesulfobacterium, Thermodesulfovibrio, Thermodesulforhabdus and Desulfomicrobium. The sulfur-oxidizing prokaryotes (SOP) belonged to α-, β- and γ-Proteobacteria, mainly represented by Roseovarius, Rhizobium, Methylobacterium, Thiobacillus and Thauera genera. In addition, the nitrate-reducing and denitrifying communities were composed of α-, β- and γ-Proteobacteria, among which Bosea, Pseudomonas, Marinobacter, Diaphorobacter and Mesorhizobium showed a relatively high abundance. The quantitative PCR results revealed that functional gene abundances of N metabolism (the napA, nirS and nosZ genes) were mostly higher than those of S metabolism (the aprA, dsrA, dsrB and soxB genes), and functional gene abundances in sample X1 were mostly higher than those in sample X2. This work provides basic data for the understanding of diversity, composition and distribution of functional microorganisms in offshore petroleum reservoir ecosystems, and also mitigation of the reservoir souring and control.

KW - Denitrifier

KW - Functional gene

KW - Nitrate-reducing bacteria

KW - Offshore petroleum reservoir

KW - Sulfate-reducing prokaryote

KW - Sulfur-oxidizing prokaryote

UR - http://www.scopus.com/inward/record.url?scp=85089417472&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2020.105057

DO - 10.1016/j.ibiod.2020.105057

M3 - 文章

AN - SCOPUS:85089417472

SN - 0964-8305

VL - 154

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 105057

ER -

Functional microorganisms involved in the sulfur and nitrogen metabolism in production water from a high-temperature offshore petroleum reservoir

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Keywords

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