Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers

Aster Hei Yiu Fung; Subramanya Rao; Wing Yui Ngan; Patrick Thabang Sekoai; Lisa Touyon; Tsoi Man Ho; Kwan Po Wong; Olivier Habimana

doi:10.1016/j.biortech.2021.125869

Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers

Aster Hei Yiu Fung, Subramanya Rao, Wing Yui Ngan, Patrick Thabang Sekoai, Lisa Touyon, Tsoi Man Ho, Kwan Po Wong, Olivier Habimana^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The possibility of breaking down cellulose-rich food waste through biofilm engineering was investigated. Six previously isolated strains from naturally degrading fruits and vegetables, screened for biofilm-forming ability and cellulolytic activity, were selected to enrich a biocarrier seeding microbial consortium. The food waste model used in this study was cabbage which was aerobically digested under repeated water rinsing and regular effluent drainage. The engineered biocarrier biofilm's functionality was evaluated by tracing microbial succession following metagenomic sequencing, quantitative PCR, scanning electron microscopy, and cellulolytic activity before and after the digestion processes. The engineered microbial consortium demonstrated superior biofilm-forming ability on biocarriers than the original microbial consortium and generally displayed a higher cellulolytic activity. The presented study provides one of the few studies of food waste aerobic digestion using engineered biofilms. Insights presented in this study could help further optimize aerobic food waste digestion.

Original language	English
Article number	125869
Journal	Bioresource Technology
Volume	341
DOIs	https://doi.org/10.1016/j.biortech.2021.125869
State	Published - Dec 2021
Externally published	Yes

Keywords

Aerobic digestion
Biofilm engineering
Cellulolytic activity
Food waste vegetable model
Selected functional wild-type strains

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.125869

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title = "Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers",

abstract = "The possibility of breaking down cellulose-rich food waste through biofilm engineering was investigated. Six previously isolated strains from naturally degrading fruits and vegetables, screened for biofilm-forming ability and cellulolytic activity, were selected to enrich a biocarrier seeding microbial consortium. The food waste model used in this study was cabbage which was aerobically digested under repeated water rinsing and regular effluent drainage. The engineered biocarrier biofilm's functionality was evaluated by tracing microbial succession following metagenomic sequencing, quantitative PCR, scanning electron microscopy, and cellulolytic activity before and after the digestion processes. The engineered microbial consortium demonstrated superior biofilm-forming ability on biocarriers than the original microbial consortium and generally displayed a higher cellulolytic activity. The presented study provides one of the few studies of food waste aerobic digestion using engineered biofilms. Insights presented in this study could help further optimize aerobic food waste digestion.",

keywords = "Aerobic digestion, Biofilm engineering, Cellulolytic activity, Food waste vegetable model, Selected functional wild-type strains",

author = "Fung, {Aster Hei Yiu} and Subramanya Rao and Ngan, {Wing Yui} and Sekoai, {Patrick Thabang} and Lisa Touyon and Ho, {Tsoi Man} and Wong, {Kwan Po} and Olivier Habimana",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2021",

month = dec,

doi = "10.1016/j.biortech.2021.125869",

language = "英语",

volume = "341",

journal = "Bioresource Technology",

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T1 - Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers

AU - Fung, Aster Hei Yiu

AU - Rao, Subramanya

AU - Ngan, Wing Yui

AU - Sekoai, Patrick Thabang

AU - Touyon, Lisa

AU - Ho, Tsoi Man

AU - Wong, Kwan Po

AU - Habimana, Olivier

PY - 2021/12

Y1 - 2021/12

N2 - The possibility of breaking down cellulose-rich food waste through biofilm engineering was investigated. Six previously isolated strains from naturally degrading fruits and vegetables, screened for biofilm-forming ability and cellulolytic activity, were selected to enrich a biocarrier seeding microbial consortium. The food waste model used in this study was cabbage which was aerobically digested under repeated water rinsing and regular effluent drainage. The engineered biocarrier biofilm's functionality was evaluated by tracing microbial succession following metagenomic sequencing, quantitative PCR, scanning electron microscopy, and cellulolytic activity before and after the digestion processes. The engineered microbial consortium demonstrated superior biofilm-forming ability on biocarriers than the original microbial consortium and generally displayed a higher cellulolytic activity. The presented study provides one of the few studies of food waste aerobic digestion using engineered biofilms. Insights presented in this study could help further optimize aerobic food waste digestion.

AB - The possibility of breaking down cellulose-rich food waste through biofilm engineering was investigated. Six previously isolated strains from naturally degrading fruits and vegetables, screened for biofilm-forming ability and cellulolytic activity, were selected to enrich a biocarrier seeding microbial consortium. The food waste model used in this study was cabbage which was aerobically digested under repeated water rinsing and regular effluent drainage. The engineered biocarrier biofilm's functionality was evaluated by tracing microbial succession following metagenomic sequencing, quantitative PCR, scanning electron microscopy, and cellulolytic activity before and after the digestion processes. The engineered microbial consortium demonstrated superior biofilm-forming ability on biocarriers than the original microbial consortium and generally displayed a higher cellulolytic activity. The presented study provides one of the few studies of food waste aerobic digestion using engineered biofilms. Insights presented in this study could help further optimize aerobic food waste digestion.

KW - Aerobic digestion

KW - Biofilm engineering

KW - Cellulolytic activity

KW - Food waste vegetable model

KW - Selected functional wild-type strains

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U2 - 10.1016/j.biortech.2021.125869

DO - 10.1016/j.biortech.2021.125869

M3 - 文章

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AN - SCOPUS:85114429229

SN - 0960-8524

VL - 341

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 125869

ER -

Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers

Abstract

Keywords

UN SDGs

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