@inbook{b79173e5f0df49cbada88e927ba2f6af,
title = "Engineering Yarrowia lipolytica for Production of Fatty Alcohols with YaliBrick Vectors",
abstract = "Biosynthesis of fatty alcohol holds great promise as substitute to replace petroleum-derived fatty alcohols to mitigate environmental concerns and reduce earth{\textquoteright}s carbon footprint. In this protocol, we detail the procedures of how to use the YaliBrick gene assembly platform to achieve modular assembly of fatty alcohol pathway in Y. lipolytica. To limit fatty alcohol oxidation, we will also describe the hydroxyurea-based protocols for the efficient disruption of POX1 gene, encoding the fatty acyl coenzyme A in Y. lipolytica, with the homologous arm about 500 bp. We envision that this chapter would improve our ability to engineer microbial cell factories for oleochemical and fatty alcohol production in oleaginous yeast species.",
keywords = "Fatty alcohol, Gene assembly, Gene deletion, Metabolic engineering, Oleochemicals, Y. lipolytica",
author = "Wanqi Sun and Zhiliang Yang and Peng Xu",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1414-3_11",
language = "英语",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "159--173",
booktitle = "Methods in Molecular Biology",
}